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-Structure paper
| タイトル | Nuclear pore permeability and fluid flow are modulated by its dilation state. |
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| ジャーナル・号・ページ | Mol Cell, Vol. 85, Issue 3, Page 537-554.e11, Year 2025 |
| 掲載日 | 2025年2月6日 |
 著者 | Patrick C Hoffmann / Hyuntae Kim / Agnieszka Obarska-Kosinska / Jan Philipp Kreysing / Eli Andino-Frydman / Sergio Cruz-León / Erica Margiotta / Lenka Cernikova / Jan Kosinski / Beata Turoňová / Gerhard Hummer / Martin Beck /  |
| PubMed 要旨 | Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate extreme changes in ...Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate extreme changes in osmolarity, to assess which role nuclear pore complexes (NPCs) play in achieving nuclear volume adaptation and relieving mechanical stress. We capitalize on the unique properties of D. discoideum to quantify fluid flow across NPCs. D. discoideum has an elaborate NPC structure in situ. Its dilation state affects NPC permeability for nucleocytosolic flow. Based on mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous flow across NPCs, which is distinct from canonically characterized modes of nucleocytoplasmic transport because of its dependence on pressure. Viral NPC blockage decreased nucleocytosolic flow. Our results may be relevant for any biological conditions that entail rapid nuclear size adaptation, including metastasizing cancer cells, migrating cells, or differentiating tissues. |
 リンク | Mol Cell / PubMed:39729993 |
| 手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
| 解像度 | 4.5 - 40.0 Å |
| 構造データ | EMDB-19137: Composite structure of the Dictyostelium discoideum nuclear pore complex in cells  EMDB-19139: Asymmetric subunit of the Dictyostelium discoideum nuclear pore complex  EMDB-19140: Inner ring segment of the Dictyostelium discoideum nuclear pore complex  EMDB-19141: Cytoplasmic ring segment of the Dictyostelium discoideum nuclear pore complex  EMDB-19142: Nuclear ring segment of the Dictyostelium discoideum nuclear pore complex  EMDB-19143: Lumenal ring segment of the Dictyostelium discoideum nuclear pore complex  EMDB-19144: Nuclear ring segment focused around the basket connection of the Dictyostelium discoideum nuclear pore complex  EMDB-19149: Composite structure of the Dictyostelium discoideum nuclear pore complex in cells - control condition  EMDB-19152: Asymmetric subunit of the Dictyostelium discoideum nuclear pore complex - control condition  EMDB-19155: Composite structure of the Dictyostelium discoideum nuclear pore complex in cells - hyperosmotic stress  EMDB-19156: Asymmetric subunit of the Dictyostelium discoideum nuclear pore complex - hyperosmotic stress  EMDB-19157: Composite structure of the Dictyostelium discoideum nuclear pore complex in cells - hypoosmotic stress  EMDB-19158: Asymmetric subunit of the Dictyostelium discoideum nuclear pore complex - hypoosmotic stress  EMDB-51989: Inner ring segment of the Dictyostelium discoideum NPC - control condition  EMDB-51990: Cytoplasmic ring segment of the Dictyostelium discoideum NPC - control condition  EMDB-51991: Nuclear ring segment of the Dictyostelium discoideum NPC - control condition  EMDB-51992: Lumenal ring segment of the Dictyostelium discoideum NPC - control condition  EMDB-51993: Nuclear ring segment focused around the basket connection of the Dictyostelium discoideum NPC - control condition  EMDB-51994: Inner ring segment of the Dictyostelium discoideum NPC - hyperosmotic stress  EMDB-51995: Cytoplasmic ring segment of the Dictyostelium discoideum NPC - hyperosmotic stress  EMDB-51996: Nuclear ring segment of the Dictyostelium discoideum NPC - hyperosmotic stress  EMDB-51997: Lumenal ring segment of the Dictyostelium discoideum NPC - hyperosmotic stress  EMDB-51998: Nuclear ring segment focused around the basket connection of the Dictyostelium discoideum NPC - hyperosmotic stress  EMDB-51999: Inner ring segment of the Dictyostelium discoideum NPC - hypoosmotic stress  EMDB-52000: Cytoplasmic ring segment of the Dictyostelium discoideum NPC - hypoosmotic stress  EMDB-52001: Nuclear ring segment of the Dictyostelium discoideum NPC - hypoosmotic stress  EMDB-52002: Lumenal ring segment of the Dictyostelium discoideum NPC - hypoosmotic stress  EMDB-52003: Nuclear ring segment focused around the basket connection of the Dictyostelium discoideum NPC - hypoosmotic stress  EMDB-52053: Cryo-tomogram of Dictyostelium discoideum cell - control condition (HL5), related to Figure 1b  EMDB-52054: Cryo-tomogram of Dictyostelium discoideum cell - hyperosmotic stress condition (+0.4 M sorbitol), related to Figure 1b  EMDB-52055: Cryo-tomogram of Dictyostelium discoideum cell - hypoosmotic stress condition (distilled water), related to Figure 1b  EMDB-52057: In situ Dictyostelium discoideum 80S ribosome - hyperosmotic stress condition  EMDB-52058: In situ Dictyostelium discoideum 80S ribosome - hypoosmotic stress condition |
| 由来 |
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 キーワード | NUCLEAR PROTEIN / Nuclear pore complex |
Dictyostelium discoideum AX2 (キイロタマホコリカビ)