EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for carbohydrate recognition by the Gal/GalNAc lectin of Entamoeba histolytica involved in host cell adhesion.
ジャーナル・号・ページ	PLoS Pathog, Vol. 22, Issue 2, Page e1013948, Year 2026
掲載日	2026年2月24日
著者	Samuel F Gérard / Christina Redfield / Matthew K Higgins /
PubMed 要旨	Intestinal amoebiasis is caused by Entamoeba histolytica, one of the deadliest human-infective parasites. Central to its pathogenicity is its binding to mucosal carbohydrates, which precedes tissue ...Intestinal amoebiasis is caused by Entamoeba histolytica, one of the deadliest human-infective parasites. Central to its pathogenicity is its binding to mucosal carbohydrates, which precedes tissue damage by trogocytosis. Carbohydrate binding is mediated by a single adhesin, the galactose/N-acetylgalactosamine (Gal/GalNAc) lectin, which is the leading vaccine candidate for amoebiasis. We present the structure of the native heterodimeric lectin, revealing an ordered core containing the light chain and the N-terminal region of the heavy chain. Structures obtained in the presence of ligand show that the Gal/GalNAc binding site is in the light chain, which adopts a β-trefoil fold found in other lectins. An elongated arm emerges from the heavy chain, which adopts multiple positions and may be modulated by sugar binding. This study reveals the molecular basis for sugar binding by the Entamoeba histolytica Gal/GalNAc lectin, a prerequisite for parasite invasion and development of intestinal disease.
リンク	PLoS Pathog / PubMed:41734235 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.818 - 3.9 Å
構造データ	51385 9gja EMDB-51385, PDB-9gja: Entamoeba histolytica Gal/GalNAc lectin, mode 1 手法: EM (単粒子) / 解像度: 3.7 Å 51386 9gjb EMDB-51386, PDB-9gjb: Entamoeba histolytica Gal/GalNAc lectin, mode 2 手法: EM (単粒子) / 解像度: 3.5 Å 51387 9gjc EMDB-51387, PDB-9gjc: Entamoeba histolytica Gal/GalNAc lectin, mode 3 手法: EM (単粒子) / 解像度: 3.9 Å 51392 9gjh EMDB-51392, PDB-9gjh: Entamoeba histolytica Gal/GalNAc lectin bound to galactose, mode 1 手法: EM (単粒子) / 解像度: 3.0 Å 51393 9gji EMDB-51393, PDB-9gji: Entamoeba histolytica Gal/GalNAc lectin bound to galactose, mode 2 手法: EM (単粒子) / 解像度: 3.4 Å 51394 9gjj EMDB-51394, PDB-9gjj: Entamoeba histolytica Gal/GalNAc lectin bound to galactose, mode 3 手法: EM (単粒子) / 解像度: 3.9 Å 51395 9gjk EMDB-51395, PDB-9gjk: Entamoeba histolytica Gal/GalNAc lectin bound to LacNAc, mode 1 手法: EM (単粒子) / 解像度: 3.3 Å 51396 9gjl EMDB-51396, PDB-9gjl: Entamoeba histolytica Gal/GalNAc lectin bound to LacNAc, mode 2 手法: EM (単粒子) / 解像度: 3.4 Å 51397 9gjm EMDB-51397, PDB-9gjm: Entamoeba histolytica Gal/GalNAc lectin bound to LacNAc, mode 3 手法: EM (単粒子) / 解像度: 3.9 Å PDB-9ged: Entamoeba histolytica Gal/GalNAc lectin heavy chain residues 808-992 手法: X-RAY DIFFRACTION / 解像度: 2.484 Å PDB-9gee: Entamoeba histolytica Gal/GalNAc lectin heavy chain residues 808-992 in the presence of galactose 手法: X-RAY DIFFRACTION / 解像度: 1.818 Å PDB-9geg: Entamoeba histolytica Gal/GalNAc lectin heavy chain residues 808-992 in the presence of GalNAc 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-9geh: Entamoeba histolytica Gal/GalNAc lectin heavy chain residues 808-992 in the presence of LacNAc 手法: X-RAY DIFFRACTION / 解像度: 2.319 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-HOH: WATER ChemComp-GAL: beta-D-galactopyranose
由来	entamoeba histolytica hm-1:imss (赤痢アメーバ) entamoeba histolytica (赤痢アメーバ)
キーワード	CELL ADHESION / Entamoeba histolytica / lectin / Gal/GalNAc / trogocytosis