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-Structure paper
タイトル | Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 106, Issue 4, Page 1063-1068, Year 2009 |
掲載日 | 2009年1月27日 |
![]() | Elizabeth Villa / Jayati Sengupta / Leonardo G Trabuco / Jamie LeBarron / William T Baxter / Tanvir R Shaikh / Robert A Grassucci / Poul Nissen / Måns Ehrenberg / Klaus Schulten / Joachim Frank / ![]() |
PubMed 要旨 | In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of ...In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.7-A cryo-electron microscopy map of the aminoacyl-tRNA x EF-Tu x GDP x kirromycin-bound Escherichia coli ribosome, together with an atomic model of the complex obtained through molecular dynamics flexible fitting. The model reveals the conformational changes in the conserved GTPase switch regions of EF-Tu that trigger hydrolysis of GTP, along with key interactions, including those between the sarcin-ricin loop and the P loop of EF-Tu, and between the effector loop of EF-Tu and a conserved region of the 16S rRNA. Our data suggest that GTP hydrolysis on EF-Tu is controlled through a hydrophobic gate mechanism. |
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手法 | EM (単粒子) |
解像度 | 6.7 Å |
構造データ | |
化合物 | ![]() ChemComp-GDP: |
由来 |
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![]() | RIBOSOME / ternary complex / flexible fitting / cryo-EM / 30S / 50S / tRNA / mRNA / EF-Tu / 70S / Ribonucleoprotein / Ribosomal protein / RNA-binding / rRNA-binding / Antibiotic resistance / Repressor / Transcription / Transcription regulation / Transcription termination / Translation regulation / tRNA-binding / Methylation / Endonuclease / Hydrolase / Nuclease / Cell membrane / Elongation factor / GTP-binding / Membrane / Nucleotide-binding / Phosphoprotein / Protein biosynthesis |