+検索条件
-Structure paper
タイトル | Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane. |
---|---|
ジャーナル・号・ページ | Nat Plants, Vol. 5, Issue 4, Page 436-446, Year 2019 |
掲載日 | 2019年4月8日 |
著者 | Anna Rast / Miroslava Schaffer / Sahradha Albert / William Wan / Stefan Pfeffer / Florian Beck / Jürgen M Plitzko / Jörg Nickelsen / Benjamin D Engel / |
PubMed 要旨 | Little is known about how the photosynthetic machinery is arranged in time and space during the biogenesis of thylakoid membranes. Using in situ cryo-electron tomography to image the three- ...Little is known about how the photosynthetic machinery is arranged in time and space during the biogenesis of thylakoid membranes. Using in situ cryo-electron tomography to image the three-dimensional architecture of the cyanobacterium Synechocystis, we observed that the tips of multiple thylakoids merge to form a substructure called the 'convergence membrane'. This high-curvature membrane comes into close contact with the plasma membrane at discrete sites. We generated subtomogram averages of 70S ribosomes and array-forming phycobilisomes, then mapped these structures onto the native membrane architecture as markers for protein synthesis and photosynthesis, respectively. This molecular localization identified two distinct biogenic regions in the thylakoid network: thylakoids facing the cytosolic interior of the cell that were associated with both marker complexes, and convergence membranes that were decorated by ribosomes but not phycobilisomes. We propose that the convergence membranes perform a specialized biogenic function, coupling the synthesis of thylakoid proteins with the integration of cofactors from the plasma membrane and the periplasmic space. |
リンク | Nat Plants / PubMed:30962530 |
手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 14.2 - 33.0 Å |
構造データ | EMDB-4599: EMDB-4600: EMDB-4601: EMDB-4602: EMDB-4603: EMDB-4604: |
由来 |
|