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-Structure paper
タイトル | Cryo-ET reveals the architecture of the polar tube invasion apparatus from microsporidian parasites. |
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ジャーナル・号・ページ | bioRxiv, Year 2024 |
掲載日 | 2024年7月13日 |
![]() | Mahrukh Usmani / Nicolas Coudray / Margot Riggi / Rishwanth Raghu / Harshita Ramchandani / Daija Bobe / Mykhailo Kopylov / Ellen D Zhong / Janet H Iwasa / Damian C Ekiert / Gira Bhabha |
PubMed 要旨 | Microsporidia are divergent fungal pathogens that employ a harpoon-like apparatus called the polar tube (PT) to invade host cells. The PT architecture and its association with neighboring organelles ...Microsporidia are divergent fungal pathogens that employ a harpoon-like apparatus called the polar tube (PT) to invade host cells. The PT architecture and its association with neighboring organelles remain poorly understood. Here, we use cryo-electron tomography to investigate the structural cell biology of the PT in dormant spores from the human-infecting microsporidian species, . Segmentation and subtomogram averaging of the PT reveal at least four layers: two protein-based layers surrounded by a membrane, and filled with a dense core. Regularly spaced protein filaments form the structural skeleton of the PT. Combining cryo-electron tomography with cellular modeling, we propose a model for the 3-dimensional organization of the polaroplast, an organelle that is continuous with the membrane layer that envelops the PT. Our results reveal the ultrastructure of the microsporidian invasion apparatus , laying the foundation for understanding infection mechanisms. |
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手法 | EM (サブトモグラム平均) |
解像度 | 20.0 - 70.0 Å |
構造データ | ![]() EMDB-45671: Subtomogram average of the Polar Tube Outer Filament layer from Encephalitozoon intestinalis microsporidian spores ![]() EMDB-45672: Subtomogram average of the Polar Tube Inner Filament layer from Encephalitozoon intestinalis microsporidian spores ![]() EMDB-45673: Subtomogram average of the Polar Tube Outer Filament Layer and Inner Filament layer from Encephalitozoon intestinalis microsporidian spores ![]() EMDB-45674: Subtomogram average of a whole Polar Tube cross-section from Encephalitozoon intestinalis microsporidian spores |
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