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-Structure paper
| タイトル | Composition and in situ structure of the cell envelope and surface layer. |
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| ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 50, Page eadr8596, Year 2024 |
| 掲載日 | 2024年12月13日 |
著者 | Hui Wang / Jiayan Zhang / Shiqing Liao / Anne M Henstra / Deborah Leon / Jonathan Erde / Joseph A Loo / Rachel R Ogorzalek Loo / Z Hong Zhou / Robert P Gunsalus / ![]() |
| PubMed 要旨 | Archaea share genomic similarities with Eukarya and cellular architectural similarities with Bacteria, though archaeal and bacterial surface layers (S-layers) differ. Using cellular cryo-electron ...Archaea share genomic similarities with Eukarya and cellular architectural similarities with Bacteria, though archaeal and bacterial surface layers (S-layers) differ. Using cellular cryo-electron tomography, we visualized the S-layer lattice surrounding , a methanogenic archaeon. Though more compact than known structures, 's S-layer is a flexible hexagonal lattice of dome-shaped tiles, uniformly spaced from both the overlying cell sheath and the underlying cell membrane. Subtomogram averaging resolved the S-layer hexamer tile at 6.4-angstrom resolution. By fitting an AlphaFold model into hexamer tiles in flat and curved conformations, we uncover intra- and intertile interactions that contribute to the S-layer's cylindrical and flexible architecture, along with a spacer extension for cell membrane attachment. cell's end plug structure, likely composed of S-layer isoforms, further highlights the uniqueness of this archaeal cell. These structural features offer advantages for methane release and reflect divergent evolutionary adaptations to environmental pressures during early microbial emergence. |
リンク | Sci Adv / PubMed:39671499 / PubMed Central |
| 手法 | EM (サブトモグラム平均) |
| 解像度 | 6.4 - 8.5 Å |
| 構造データ | ![]() EMDB-45366: M. hungatei S-layer hexamer (C6) ![]() EMDB-45367: M. hungatei SLP hexamer in curved conformation (C2) |
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Methanospirillum hungatei JF-1 (古細菌)