EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Assembly of the bacterial ribosome with circularly permuted rRNA.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 52, Issue 18, Page 11254-11265, Year 2024
掲載日	2024年10月14日
著者	Xiyu Dong / Kai Sheng / Luca F R Gebert / Sriram Aiyer / Ian J MacRae / Dmitry Lyumkis / James R Williamson /
PubMed 要旨	Co-transcriptional assembly is an integral feature of the formation of RNA-protein complexes that mediate translation. For ribosome synthesis, prior studies have indicated that the strict order of ...Co-transcriptional assembly is an integral feature of the formation of RNA-protein complexes that mediate translation. For ribosome synthesis, prior studies have indicated that the strict order of transcription of rRNA domains may not be obligatory during bacterial ribosome biogenesis, since a series of circularly permuted rRNAs are viable. In this work, we report the structural insights into assembly of the bacterial ribosome large subunit (LSU) based on cryo-EM density maps of intermediates that accumulate during in vitro ribosome synthesis using a set of circularly permuted (CiPer) rRNAs. The observed ensemble of 23 resolved ribosome large subunit intermediates reveals conserved assembly routes with an underlying hierarchy among cooperative assembly blocks. There are intricate interdependencies for the formation of key structural rRNA helices revealed from the circular permutation of rRNA. While the order of domain synthesis is not obligatory, the order of domain association does appear to proceed with a particular order, likely due to the strong evolutionary pressure on efficient ribosome synthesis. This work reinforces the robustness of the known assembly hierarchy of the bacterial large ribosomal subunit and offers a coherent view of how efficient assembly of CiPer rRNAs can be understood in that context.
リンク	Nucleic Acids Res / PubMed:39036963 / PubMed Central
手法	EM (単粒子)
解像度	4.8 - 8.7 Å
構造データ	EMDB-43844: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class B 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-43845: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class C1 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-43846: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class C2 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-43847: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class E2 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-43848: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class G 手法: EM (単粒子) / 解像度: 6.8 Å EMDB-43849: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP45 Class E1 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-43850: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63 Class C 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-43851: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63 Class E2 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-43852: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63 Class E1 手法: EM (単粒子) / 解像度: 5.7 Å EMDB-43853: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63 Class D 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-43854: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63 Class B 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-43856: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class E 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-43857: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class C2 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-43858: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class B 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-43859: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class C3 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-43860: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class D1 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-43861: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class C1 手法: EM (単粒子) / 解像度: 8.1 Å EMDB-43862: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class C4 手法: EM (単粒子) / 解像度: 7.3 Å EMDB-43863: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP63(+PS) Class D2 手法: EM (単粒子) / 解像度: 8.7 Å EMDB-43864: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP78 Class B 手法: EM (単粒子) / 解像度: 8.7 Å EMDB-43865: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP78 Class C1 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-43866: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP78 Class C2 手法: EM (単粒子) / 解像度: 8.0 Å EMDB-43867: Structure of Circularly Permuted 50S Ribosomal Subunit Assembly Intermediate - CP78 Class C3 手法: EM (単粒子) / 解像度: 5.6 Å
由来	Escherichia coli (大腸菌)