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-Structure paper
タイトル | Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines. |
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ジャーナル・号・ページ | NPJ Vaccines, Vol. 9, Issue 1, Page 74, Year 2024 |
掲載日 | 2024年4月6日 |
著者 | Iván Del Moral-Sánchez / Edmund G Wee / Yuejiao Xian / Wen-Hsin Lee / Joel D Allen / Alba Torrents de la Peña / Rebeca Fróes Rocha / James Ferguson / André N León / Sylvie Koekkoek / Edith E Schermer / Judith A Burger / Sanjeev Kumar / Robby Zwolsman / Mitch Brinkkemper / Aafke Aartse / Dirk Eggink / Julianna Han / Meng Yuan / Max Crispin / Gabriel Ozorowski / Andrew B Ward / Ian A Wilson / Tomáš Hanke / Kwinten Sliepen / Rogier W Sanders / |
PubMed 要旨 | Recombinant native-like HIV-1 envelope glycoprotein (Env) trimers are used in candidate vaccines aimed at inducing broadly neutralizing antibodies. While state-of-the-art SOSIP or single-chain Env ...Recombinant native-like HIV-1 envelope glycoprotein (Env) trimers are used in candidate vaccines aimed at inducing broadly neutralizing antibodies. While state-of-the-art SOSIP or single-chain Env designs can be expressed as native-like trimers, undesired monomers, dimers and malformed trimers that elicit non-neutralizing antibodies are also formed, implying that these designs could benefit from further modifications for gene-based vaccination approaches. Here, we describe the triple tandem trimer (TTT) design, in which three Env protomers are genetically linked in a single open reading frame and express as native-like trimers. Viral vectored Env TTT induced similar neutralization titers but with a higher proportion of trimer-specific responses. The TTT design was also applied to generate influenza hemagglutinin (HA) trimers without the need for trimerization domains. Additionally, we used TTT to generate well-folded chimeric Env and HA trimers that harbor protomers from three different strains. In summary, the TTT design is a useful platform for the design of HIV-1 Env and influenza HA immunogens for a multitude of vaccination strategies. |
リンク | NPJ Vaccines / PubMed:38582771 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 5.75 - 28.52 Å |
構造データ | EMDB-42464: chEnv TTT protein in complex with 43A2 Fab EMDB-42468: chEnv TTT protein in complex with CM01A Fab EMDB-43664: Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines EMDB-43665: Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (cH125 TTT) EMDB-43666: Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (H2/1 GCN4) EMDB-43668: Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (H5/1 GCN4) EMDB-43669: Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines. H5 GCN4 PDB-8tgo: |
化合物 | ChemComp-NAG: |
由来 |
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キーワード | VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / HIV (ヒト免疫不全ウイルス) / envelope (封筒) / antibody (抗体) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) |