EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Differences between bacteria and eukaryotes in clamp loader mechanism, a conserved process underlying DNA replication.
ジャーナル・号・ページ	J Biol Chem, Vol. 300, Issue 4, Page 107166, Year 2024
掲載日	2024年3月14日
著者	Jacob T Landeck / Joshua Pajak / Emily K Norman / Emma L Sedivy / Brian A Kelch /
PubMed 要旨	Clamp loaders are pentameric ATPases that place circular sliding clamps onto DNA, where they function in DNA replication and genome integrity. The central activity of a clamp loader is the opening of ...Clamp loaders are pentameric ATPases that place circular sliding clamps onto DNA, where they function in DNA replication and genome integrity. The central activity of a clamp loader is the opening of the ring-shaped sliding clamp and the subsequent binding to primer-template (p/t)-junctions. The general architecture of clamp loaders is conserved across all life, suggesting that their mechanism is retained. Recent structural studies of the eukaryotic clamp loader replication factor C (RFC) revealed that it functions using a crab-claw mechanism, where clamp opening is coupled to a massive conformational change in the loader. Here we investigate the clamp loading mechanism of the Escherichia coli clamp loader at high resolution using cryo-electron microscopy. We find that the E. coli clamp loader opens the clamp using a crab-claw motion at a single pivot point, whereas the eukaryotic RFC loader uses motions distributed across the complex. Furthermore, we find clamp opening occurs in multiple steps, starting with a partly open state with a spiral conformation, and proceeding to a wide open clamp in a surprising planar geometry. Finally, our structures in the presence of p/t-junctions illustrate how the clamp closes around p/t-junctions and how the clamp loader initiates release from the loaded clamp. Our results reveal mechanistic distinctions in a macromolecular machine that is conserved across all domains of life.
リンク	J Biol Chem / PubMed:38490435 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 7.7 Å
構造データ	43094 8val EMDB-43094, PDB-8val: Structure of the E. coli clamp loader bound to the beta clamp in a Open-DNAp/t conformation 手法: EM (単粒子) / 解像度: 3.7 Å 43095 8vam EMDB-43095, PDB-8vam: Structure of the E. coli clamp loader bound to the beta clamp in a Semi-Open conformation 手法: EM (単粒子) / 解像度: 3.9 Å 43096 8van EMDB-43096, PDB-8van: Structure of the E. coli clamp loader bound to the beta clamp in an Initial-Binding conformation 手法: EM (単粒子) / 解像度: 7.7 Å 43098 8vap EMDB-43098, PDB-8vap: Structure of the E. coli clamp loader bound to the beta clamp in a Fully-Open conformation 手法: EM (単粒子) / 解像度: 3.0 Å 43099 8vaq EMDB-43099, PDB-8vaq: Structure of the E. coli clamp loader bound to the beta clamp in a Closed-DNA1 conformation 手法: EM (単粒子) / 解像度: 3.8 Å 43100 8var EMDB-43100, PDB-8var: Structure of the E. coli clamp loader bound to the beta clamp in a Closed-DNA2 conformation 手法: EM (単粒子) / 解像度: 3.9 Å 43101 8vas EMDB-43101, PDB-8vas: Structure of the E. coli clamp loader bound to the beta clamp in an Altered-Collar conformation 手法: EM (単粒子) / 解像度: 3.8 Å 43102 8vat EMDB-43102, PDB-8vat: Structure of the E. coli clamp loader bound to the beta clamp in a Open-RNAp/t conformation 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	escherichia coli (大腸菌) synthetic construct (人工物)
キーワード	REPLICATION (DNA複製) / TRANSFERASE/DNA / Bacterial Clamp Loader Complex / TRANSFERASE-DNA complex / TRANSFERASE (転移酵素) / REPLICATION/DNA / REPLICATION-DNA complex