EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of directional switching by the bacterial flagellum.
ジャーナル・号・ページ	Nat Microbiol, Vol. 9, Issue 5, Page 1282-1292, Year 2024
掲載日	2024年3月8日
著者	Steven Johnson / Justin C Deme / Emily J Furlong / Joseph J E Caesar / Fabienne F V Chevance / Kelly T Hughes / Susan M Lea /
PubMed 要旨	The bacterial flagellum is a macromolecular protein complex that harvests energy from uni-directional ion flow across the inner membrane to power bacterial swimming via rotation of the flagellar ...The bacterial flagellum is a macromolecular protein complex that harvests energy from uni-directional ion flow across the inner membrane to power bacterial swimming via rotation of the flagellar filament. Rotation is bi-directional, with binding of a cytoplasmic chemotactic response regulator controlling reversal, though the structural and mechanistic bases for rotational switching are not well understood. Here we present cryoelectron microscopy structures of intact Salmonella flagellar basal bodies (3.2-5.5 Å), including the cytoplasmic C-ring complexes required for power transmission, in both counter-clockwise and clockwise rotational conformations. These reveal 180° movements of both the N- and C-terminal domains of the FliG protein, which, when combined with a high-resolution cryoelectron microscopy structure of the MotAB stator, show that the stator shifts from the outside to the inside of the C-ring. This enables rotational switching and reveals how uni-directional ion flow across the inner membrane is used to accomplish bi-directional rotation of the flagellum.
リンク	Nat Microbiol / PubMed:38459206
手法	EM (単粒子)
解像度	2.4 - 5.4 Å
構造データ	42139 8ucs EMDB-42139, PDB-8ucs: Cryo-EM structure of the flagellar MotAB stator bound to FliG 手法: EM (単粒子) / 解像度: 2.4 Å 42376 8umd EMDB-42376, PDB-8umd: Cryo-EM structure of a single subunit of a Counterclockwise-locked form of the Salmonella enterica Typhimurium flagellar C-ring. 手法: EM (単粒子) / 解像度: 3.6 Å 42387 8umx EMDB-42387, PDB-8umx: Cryo-EM structure of a single subunit of a Clockwise-locked form of the Salmonella enterica Typhimurium flagellar C-ring. 手法: EM (単粒子) / 解像度: 4.0 Å 42439 8uox EMDB-42439, PDB-8uox: Cryo-EM structure of a Counterclockwise locked form of the Salmonella enterica Typhimurium flagellar C-ring, with C34 symmetry applied 手法: EM (単粒子) / 解像度: 4.6 Å 42451 8upl EMDB-42451, PDB-8upl: Cryo-EM structure of a Clockwise locked form of the Salmonella enterica Typhimurium flagellar C-ring, with C34 symmetry applied 手法: EM (単粒子) / 解像度: 5.4 Å
化合物	ChemComp-HOH: WATER
由来	clostridium sporogenes (バクテリア) salmonella enterica subsp. enterica serovar typhimurium (サルモネラ菌)
キーワード	MEMBRANE PROTEIN / flagella chemotaxis motility type III secretion system / MOTOR PROTEIN / Flagella / C-ring / Salmonella / motor / rotation