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-Structure paper
タイトル | Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit. |
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ジャーナル・号・ページ | EMBO J, Vol. 37, Issue 7, Year 2018 |
掲載日 | 2018年4月3日 |
著者 | Alain Scaiola / Cohue Peña / Melanie Weisser / Daniel Böhringer / Marc Leibundgut / Purnima Klingauf-Nerurkar / Stefan Gerhardy / Vikram Govind Panse / Nenad Ban / |
PubMed 要旨 | Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron ...Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron microscopy (cryo-EM), we have determined the structure of a yeast cytoplasmic pre-40S particle in complex with Enp1, Ltv1, Rio2, Tsr1, and Pno1 assembly factors poised to initiate final maturation. The structure reveals that the pre-rRNA adopts a highly distorted conformation of its 3' major and 3' minor domains stabilized by the binding of the assembly factors. This observation is consistent with a mechanism that involves concerted release of the assembly factors orchestrated by the folding of the rRNA in the head of the pre-40S subunit during the final stages of maturation. Our results provide a structural framework for the coordination of the final maturation events that drive a pre-40S particle toward the mature form capable of engaging in translation. |
リンク | EMBO J / PubMed:29459436 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 4.3 Å |
構造データ | EMDB-4214, PDB-6fai: EMDB-4215: EMDB-4216: EMDB-4217: EMDB-4218: |
化合物 | ChemComp-ZN: ChemComp-MG: |
由来 |
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キーワード | RIBOSOME / pre-40S ribosme / cryo-EM / ribosome assembly / ribosomes / ribosome biogenesis |