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-Structure paper
タイトル | Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 379, Year 2024 |
掲載日 | 2024年1月9日 |
![]() | Nicolas Dos Santos Pacheco / Albert Tell I Puig / Amandine Guérin / Matthew Martinez / Bohumil Maco / Nicolò Tosetti / Estefanía Delgado-Betancourt / Matteo Lunghi / Boris Striepen / Yi-Wei Chang / Dominique Soldati-Favre / ![]() ![]() |
PubMed 要旨 | In Apicomplexa, rhoptry discharge is essential for invasion and involves an apical vesicle (AV) docking one or two rhoptries to a macromolecular secretory apparatus. Toxoplasma gondii is armed with ...In Apicomplexa, rhoptry discharge is essential for invasion and involves an apical vesicle (AV) docking one or two rhoptries to a macromolecular secretory apparatus. Toxoplasma gondii is armed with 10-12 rhoptries and 5-6 microtubule-associated vesicles (MVs) presumably for iterative rhoptry discharge. Here, we have addressed the localization and functional significance of two intraconoidal microtubule (ICMT)-associated proteins instrumental for invasion. Mechanistically, depletion of ICMAP2 leads to a dissociation of the ICMTs, their detachment from the conoid and dispersion of MVs and rhoptries. ICMAP3 exists in two isoforms that contribute to the control of the ICMTs length and the docking of the two rhoptries at the AV, respectively. This study illuminates the central role ICMTs play in scaffolding the discharge of multiple rhoptries. This process is instrumental for virulence in the mouse model of infection and in addition promotes sterile protection against T. gondii via the release of key effectors inducing immunity. |
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手法 | EM (トモグラフィー) |
構造データ | ![]() EMDB-42118: Toxoplasma gondii apical end with ICMAP1 conditionally knocked down ![]() EMDB-42119: Toxoplasma gondii apical end with ICMAP2 conditionally knocked down ![]() EMDB-42120: Toxoplasma gondii apical end with ICMAP3i conditionally knocked down ![]() EMDB-42121: Toxoplasma gondii apical end with ICMAP3ii conditionally knocked down |
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