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-Structure paper
タイトル | Removal of type IV pili by a small RNA virus. |
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ジャーナル・号・ページ | Science, Vol. 384, Issue 6691, Page eadl0635, Year 2024 |
掲載日 | 2024年4月5日 |
著者 | Jirapat Thongchol / Zihao Yu / Laith Harb / Yiruo Lin / Matthias Koch / Matthew Theodore / Utkarsh Narsaria / Joshua Shaevitz / Zemer Gitai / Yinghao Wu / Junjie Zhang / Lanying Zeng / |
PubMed 要旨 | The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through ...The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics. |
リンク | Science / PubMed:38574145 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.6 - 7.9 Å |
構造データ | EMDB-41625, PDB-8tum: EMDB-41632: Asymmetric reconstruction of mature PP7 virions EMDB-41633, PDB-8tuw: EMDB-41675: The original consensus map of PP7 binding to T4P from PAO1 EMDB-41780: Composite map of PP7 binding to type IV pilus from PAO1 |
由来 |
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キーワード | MEMBRANE PROTEIN / Type IV pilus / T4P / PAO1 / VIRAL PROTEIN / Maturation protein / PP7 / pepevirus |