EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 110, Issue 48, Page E4638-E4647, Year 2013
掲載日	2013年11月26日
著者	Vijaykumar Karuppiah / Richard F Collins / Angela Thistlethwaite / Ya Gao / Jeremy P Derrick /
PubMed 要旨	Type IV pili are long fibers that are assembled by polymerization of a major pilin protein in the periplasm of a wide range of bacteria and archaea. They play crucial roles in pathogenesis, DNA ...Type IV pili are long fibers that are assembled by polymerization of a major pilin protein in the periplasm of a wide range of bacteria and archaea. They play crucial roles in pathogenesis, DNA transformation, and motility, and are capable of rapid retraction, generating powerful motor forces. PilN and PilO are integral inner membrane proteins that are essential for type IV pilus formation. Here, we show that PilN and PilO from Thermus thermophilus can be isolated as a complex with PilM, a cytoplasmic protein with structural similarities to the cytoskeletal protein MreB. The crystal structure of the periplasmic portion of PilN forms a homodimer with an extensive, conserved interaction interface. We conducted serial 3D reconstructions by electron microscopy of PilMN, PilMNO, and PilMNO bound to the major pilin protein PilA4, to chart the assembly of the inner membrane pilus biogenesis platform. PilN drives the dimerization of the PilMN complex with a stoichiometry of 2:2; binding of two PilO monomers then causes the PilN periplasmic domains to dissociate. Finally, two PilA4 monomers bind to the periplasmic domains of PilN and PilO, to generate a T-shaped complex that is primed for addition of the pilin to the nascent pilus fiber. Docking of structures for PilM, PilN, PilO, and PilA4 into the electron density maps of the transmembrane complexes was used to generate a sequence of molecular structures that chart the initial events in type IV pilus formation, and provide structural information on the early events in this important secretion process.
リンク	Proc Natl Acad Sci U S A / PubMed:24218553 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.7 - 27.0 Å
構造データ	EMDB-4157: PilMNO complex from Thermus thermophilus 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-4158: PilMN complex from Thermus thermophilus 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-4159: PilMNOA complex from Thermus thermophilus 手法: EM (単粒子) / 解像度: 25.0 Å PDB-4bhq: Structure of the periplasmic domain of the PilN type IV pilus biogenesis protein from Thermus thermophilus 手法: X-RAY DIFFRACTION / 解像度: 2.05 Å PDB-4bhr: Structure of the TTHA1221 type IV pilin protein from Thermus thermophilus 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン
由来	Thermus thermophilus HB8 (バクテリア) thermus thermophilus (バクテリア)
キーワード	CELL ADHESION / SECRETION