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-Structure paper
タイトル | Implications of a multiscale structure of the yeast nuclear pore complex. |
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ジャーナル・号・ページ | Mol Cell, Vol. 83, Issue 18, Page 3283-33302.e5, Year 2023 |
掲載日 | 2023年9月21日 |
著者 | Christopher W Akey / Ignacia Echeverria / Christna Ouch / Ilona Nudelman / Yi Shi / Junjie Wang / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Michael P Rout / |
PubMed 要旨 | Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from ...Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution. |
リンク | Mol Cell / PubMed:37738963 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 6.6 - 31.7 Å |
構造データ | EMDB-41114: Nuclear double outer ring of the yeast NPC EMDB-41116: Lumenal ring of the isolated yeast NPC EMDB-41117, PDB-8t9l: EMDB-41119: Cytoplasmic outer ring of the yeast NPC EMDB-41120: Central transporter/plug yeast NPC EMDB-41121: Double outer ring connectors of the yeast NPC EMDB-41122: Connectors in cytoplasmic outer ring of yeast NPC EMDB-41123: Composite multiscale structure of the yeast NPC EMDB-41285, PDB-8tie: EMDB-41300, PDB-8tj5: |
由来 |
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キーワード | TRANSPORT PROTEIN / nuclear pore complex / nucleocytoplasmic transport / nucleoporin / membrane protein / translocase |