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-Structure paper
タイトル | Structural insights into vesicular monoamine storage and drug interactions. |
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ジャーナル・号・ページ | Nature, Vol. 629, Issue 8010, Page 235-243, Year 2024 |
掲載日 | 2024年3月18日 |
著者 | Jin Ye / Huaping Chen / Kaituo Wang / Yi Wang / Aaron Ammerman / Samjhana Awasthi / Jinbin Xu / Bin Liu / Weikai Li / |
PubMed 要旨 | Biogenic monoamines-vital transmitters orchestrating neurological, endocrinal and immunological functions-are stored in secretory vesicles by vesicular monoamine transporters (VMATs) for controlled ...Biogenic monoamines-vital transmitters orchestrating neurological, endocrinal and immunological functions-are stored in secretory vesicles by vesicular monoamine transporters (VMATs) for controlled quantal release. Harnessing proton antiport, VMATs enrich monoamines around 10,000-fold and sequester neurotoxicants to protect neurons. VMATs are targeted by an arsenal of therapeutic drugs and imaging agents to treat and monitor neurodegenerative disorders, hypertension and drug addiction. However, the structural mechanisms underlying these actions remain unclear. Here we report eight cryo-electron microscopy structures of human VMAT1 in unbound form and in complex with four monoamines (dopamine, noradrenaline, serotonin and histamine), the Parkinsonism-inducing MPP, the psychostimulant amphetamine and the antihypertensive drug reserpine. Reserpine binding captures a cytoplasmic-open conformation, whereas the other structures show a lumenal-open conformation stabilized by extensive gating interactions. The favoured transition to this lumenal-open state contributes to monoamine accumulation, while protonation facilitates the cytoplasmic-open transition and concurrently prevents monoamine binding to avoid unintended depletion. Monoamines and neurotoxicants share a binding pocket that possesses polar sites for specificity and a wrist-and-fist shape for versatility. Variations in this pocket explain substrate preferences across the SLC18 family. Overall, these structural insights and supporting functional studies elucidate the mechanism of vesicular monoamine transport and provide the basis to develop therapeutics for neurodegenerative diseases and substance abuse. |
リンク | Nature / PubMed:38499039 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.3 - 3.7 Å |
構造データ | EMDB-41235, PDB-8tgg: EMDB-41236, PDB-8tgh: EMDB-41237, PDB-8tgi: EMDB-41238, PDB-8tgj: EMDB-41239, PDB-8tgk: EMDB-41240, PDB-8tgl: EMDB-41241, PDB-8tgm: EMDB-41242, PDB-8tgn: |
化合物 | ChemComp-WRF: ChemComp-YHR: ChemComp-1WE: ChemComp-LDP: ChemComp-HSM: ChemComp-LNR: ChemComp-SRO: |
由来 |
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キーワード | MEMBRANE PROTEIN / VMAT / SLC18 / vascular monoamine transporter / monoamines / neurotransmitters / MPP+ / amphetamine / dopamine / reserpine / histamine / norepinephrine / serotonin |