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-Structure paper
タイトル | Poly(ADP-ribosyl)ation enhances nucleosome dynamics and organizes DNA damage repair components within biomolecular condensates. |
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ジャーナル・号・ページ | Mol Cell, Vol. 84, Issue 3, Page 429-446.e17, Year 2024 |
掲載日 | 2024年2月1日 |
著者 | Michael L Nosella / Tae Hun Kim / Shuya Kate Huang / Robert W Harkness / Monica Goncalves / Alisia Pan / Maria Tereshchenko / Siavash Vahidi / John L Rubinstein / Hyun O Lee / Julie D Forman-Kay / Lewis E Kay / |
PubMed 要旨 | Nucleosomes, the basic structural units of chromatin, hinder recruitment and activity of various DNA repair proteins, necessitating modifications that enhance DNA accessibility. Poly(ADP-ribosyl) ...Nucleosomes, the basic structural units of chromatin, hinder recruitment and activity of various DNA repair proteins, necessitating modifications that enhance DNA accessibility. Poly(ADP-ribosyl)ation (PARylation) of proteins near damage sites is an essential initiation step in several DNA-repair pathways; however, its effects on nucleosome structural dynamics and organization are unclear. Using NMR, cryoelectron microscopy (cryo-EM), and biochemical assays, we show that PARylation enhances motions of the histone H3 tail and DNA, leaving the configuration of the core intact while also stimulating nuclease digestion and ligation of nicked nucleosomal DNA by LIG3. PARylation disrupted interactions between nucleosomes, preventing self-association. Addition of LIG3 and XRCC1 to PARylated nucleosomes generated condensates that selectively partition DNA repair-associated proteins in a PAR- and phosphorylation-dependent manner in vitro. Our results establish that PARylation influences nucleosomes across different length scales, extending from the atom-level motions of histone tails to the mesoscale formation of condensates with selective compositions. |
リンク | Mol Cell / PubMed:38215753 |
手法 | EM (単粒子) |
解像度 | 3.3 - 3.7 Å |
構造データ | EMDB-41178: Cryo-EM map of the PARylated nucleosome core particle in 5 mM KCl with local resolution values EMDB-41182: Cryo-EM map of the Unmodified nucleosome core particle in 100 mM KCl with local resolution values EMDB-41183: Cryo-EM map of the PARylated nucleosome core particle in 100 mM KCl with local resolution values EMDB-41184: Cryo-EM map of the Unmodified nucleosome core particle in 5 mM KCl with local resolution values |
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