+検索条件
-Structure paper
タイトル | Open structure and gating of the Arabidopsis mechanosensitive ion channel MSL10. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 6284, Year 2023 |
掲載日 | 2023年10月7日 |
著者 | Jingying Zhang / Grigory Maksaev / Peng Yuan / |
PubMed 要旨 | Plants are challenged by drastically different osmotic environments during growth and development. Adaptation to these environments often involves mechanosensitive ion channels that can detect and ...Plants are challenged by drastically different osmotic environments during growth and development. Adaptation to these environments often involves mechanosensitive ion channels that can detect and respond to mechanical force. In the model plant Arabidopsis thaliana, the mechanosensitive channel MSL10 plays a crucial role in hypo-osmotic shock adaptation and programmed cell death induction, but the molecular basis of channel function remains poorly understood. Here, we report a structural and electrophysiological analysis of MSL10. The cryo-electron microscopy structures reveal a distinct heptameric channel assembly. Structures of the wild-type channel in detergent and lipid environments, and in the absence of membrane tension, capture an open conformation. Furthermore, structural analysis of a non-conductive mutant channel demonstrates that reorientation of phenylalanine side chains alone, without main chain rearrangements, may generate the hydrophobic gate. Together, these results reveal a distinct gating mechanism and advance our understanding of mechanotransduction. |
リンク | Nat Commun / PubMed:37805510 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.5 - 3.7 Å |
構造データ | EMDB-41164, PDB-8tdj: EMDB-41165, PDB-8tdk: EMDB-41166, PDB-8tdl: EMDB-41168, PDB-8tdm: |
由来 |
|
キーワード | TRANSPORT PROTEIN / ion channels / mechanosensitive channels / heptamer / Arabidopsis thaliana |