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-Structure paper
タイトル | Dual receptor-sites reveal the structural basis for hyperactivation of sodium channels by poison-dart toxin batrachotoxin. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 2306, Year 2024 |
掲載日 | 2024年3月14日 |
![]() | Lige Tonggu / Goragot Wisedchaisri / Tamer M Gamal El-Din / Michael J Lenaeus / Matthew M Logan / Tatsuya Toma / Justin Du Bois / Ning Zheng / William A Catterall / ![]() ![]() |
PubMed 要旨 | The poison dart toxin batrachotoxin is exceptional for its high potency and toxicity, and for its multifaceted modification of the function of voltage-gated sodium channels. By using cryogenic ...The poison dart toxin batrachotoxin is exceptional for its high potency and toxicity, and for its multifaceted modification of the function of voltage-gated sodium channels. By using cryogenic electron microscopy, we identify two homologous, but nonidentical receptor sites that simultaneously bind two molecules of toxin, one at the interface between Domains I and IV, and the other at the interface between Domains III and IV of the cardiac sodium channel. Together, these two bound toxin molecules stabilize α/π helical conformation in the S6 segments that gate the pore, and one of the bound BTX-B molecules interacts with the crucial Lys1421 residue that is essential for sodium conductance and selectivity via an apparent water-bridged hydrogen bond. Overall, our structure provides insight into batrachotoxin's potency, efficacy, and multifaceted functional effects on voltage-gated sodium channels via a dual receptor site mechanism. |
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手法 | EM (単粒子) |
解像度 | 3.3 Å |
構造データ | EMDB-41071, PDB-8t6l: |
化合物 | ![]() ChemComp-NAG: ![]() ChemComp-LBN: ![]() ChemComp-Y01: ![]() ChemComp-9Z9: ![]()
ChemComp-YIJ: ![]() ChemComp-HOH: |
由来 |
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![]() | MEMBRANE PROTEIN / Ion channel / Sodium channel / Voltage-gated channel / Sodium transport |