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-Structure paper
タイトル | Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission. |
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ジャーナル・号・ページ | Dev Cell, Vol. 59, Issue 14, Page 1783-11793.e5, Year 2024 |
掲載日 | 2024年7月22日 |
![]() | John R Jimah / Nidhi Kundu / Abigail E Stanton / Kem A Sochacki / Bertram Canagarajah / Lieza Chan / Marie-Paule Strub / Huaibin Wang / Justin W Taraska / Jenny E Hinshaw / ![]() |
PubMed 要旨 | Dynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the ...Dynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the plasma membrane. Although atomic models of the dynamin helical polymer bound to guanosine triphosphate (GTP) analogs define earlier stages of membrane constriction, there are no atomic models of the assembled state post-GTP hydrolysis. Here, we used cryo-EM methods to determine atomic structures of the dynamin helical polymer assembled on lipid tubules, akin to necks of budding endocytic vesicles, in a guanosine diphosphate (GDP)-bound, super-constricted state. In this state, dynamin is assembled as a 2-start helix with an inner lumen of 3.4 nm, primed for spontaneous fission. Additionally, by cryo-electron tomography, we trapped dynamin helical assemblies within HeLa cells using the GTPase-defective dynamin K44A mutant and observed diverse dynamin helices, demonstrating that dynamin can accommodate a range of assembled complexes in cells that likely precede membrane fission. |
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手法 | EM (らせん対称) |
解像度 | 2.84 - 3.97 Å |
構造データ | EMDB-40861, PDB-8sxz: EMDB-40901, PDB-8sz4: EMDB-40902, PDB-8sz7: EMDB-40903, PDB-8sz8: EMDB-40942, PDB-8t0k, PDB-8tym: EMDB-40946, PDB-8t0r: |
化合物 | ![]() ChemComp-GDP: ![]() ChemComp-MG: |
由来 |
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![]() | HYDROLASE / dynamin / membrane / fission / lipid / tubule / scission / endocytosis |