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-Structure paper
タイトル | An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. |
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ジャーナル・号・ページ | Science, Vol. 353, Issue 6298, Page 506-508, Year 2016 |
掲載日 | 2016年7月29日 |
著者 | Florian K M Schur / Martin Obr / Wim J H Hagen / William Wan / Arjen J Jakobi / Joanna M Kirkpatrick / Carsten Sachse / Hans-Georg Kräusslich / John A G Briggs / |
PubMed 要旨 | Immature HIV-1 assembles at and buds from the plasma membrane before proteolytic cleavage of the viral Gag polyprotein induces structural maturation. Maturation can be blocked by maturation ...Immature HIV-1 assembles at and buds from the plasma membrane before proteolytic cleavage of the viral Gag polyprotein induces structural maturation. Maturation can be blocked by maturation inhibitors (MIs), thereby abolishing infectivity. The CA (capsid) and SP1 (spacer peptide 1) region of Gag is the key regulator of assembly and maturation and is the target of MIs. We applied optimized cryo-electron tomography and subtomogram averaging to resolve this region within assembled immature HIV-1 particles at 3.9 angstrom resolution and built an atomic model. The structure reveals a network of intra- and intermolecular interactions mediating immature HIV-1 assembly. The proteolytic cleavage site between CA and SP1 is inaccessible to protease. We suggest that MIs prevent CA-SP1 cleavage by stabilizing the structure, and MI resistance develops by destabilizing CA-SP1. |
リンク | Science / PubMed:27417497 |
手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 3.9 - 4.5 Å |
構造データ | EMDB-4015: A 3.9 Angstrom structure of HIV-1 CA-SP1 assembled in presence of Bevirimat EMDB-4016: EMDB-4017: EMDB-4018: EMDB-4019: EMDB-4020: |
由来 |
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キーワード | VIRAL PROTEIN / HIV-1 capsid SP1 |