EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure and molecular mechanism of the jasmonic acid transporter ABCG16.
ジャーナル・号・ページ	Nat Plants, Vol. 10, Issue 12, Page 2052-2061, Year 2024
掲載日	2024年11月4日
著者	Ning An / Xiaowei Huang / Zhao Yang / Minhua Zhang / Miaolian Ma / Fang Yu / Lianyan Jing / Boya Du / Yong-Fei Wang / Xue Zhang / Peng Zhang /
PubMed 要旨	Jasmonates (JAs) are a class of oxylipin phytohormones including jasmonic acid (JA) and derivatives that regulate plant growth, development and biotic and abiotic stress. A number of transporters ...Jasmonates (JAs) are a class of oxylipin phytohormones including jasmonic acid (JA) and derivatives that regulate plant growth, development and biotic and abiotic stress. A number of transporters have been identified to be responsible for the cellular and subcellular translocation of JAs. However, the mechanistic understanding of how these transporters specifically recognize and transport JAs is scarce. Here we determined the cryogenic electron microscopy structure of JA exporter AtABCG16 in inward-facing apo, JA-bound and occluded conformations, and outward-facing post translocation conformation. AtABCG16 structure forms a homodimer, and each monomer contains a nucleotide-binding domain, a transmembrane domain and an extracellular domain. Structural analyses together with biochemical and plant physiological experiments revealed the molecular mechanism by which AtABCG16 specifically recognizes and transports JA. Structural analyses also revealed that AtABCG16 features a unique bifurcated substrate translocation pathway, which is composed of two independent substrate entrances, two substrate-binding pockets and a shared apoplastic cavity. In addition, residue Phe608 from each monomer is disclosed to function as a gate along the translocation pathway controlling the accessing of substrate JA from the cytoplasm or apoplast. Based on the structural and biochemical analyses, a working model of AtABCG16-mediated JA transport is proposed, which diversifies the molecular mechanisms of ABC transporters.
リンク	Nat Plants / PubMed:39496849
手法	EM (単粒子)
解像度	2.38 - 3.32 Å
構造データ	37836 8wtm EMDB-37836, PDB-8wtm: Cryo-EM structure of jasmonic acid transporter ABCG16 bound to JA 手法: EM (単粒子) / 解像度: 2.65 Å 37837 8wtn EMDB-37837, PDB-8wtn: Cryo-EM structure of jasmonic acid transporter ABCG16 in occluded conformation 手法: EM (単粒子) / 解像度: 2.95 Å 37838 8wto EMDB-37838, PDB-8wto: Cryo-EM structure of jasmonic acid transporter ABCG16 in outward conformation 手法: EM (単粒子) / 解像度: 2.38 Å 37839 8wtp EMDB-37839, PDB-8wtp: Cryo-EM structure of jasmonic acid transporter ABCG16 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-37840: Cryo-EM structure of AtABCG16 手法: EM (単粒子) / 解像度: 3.32 Å EMDB-39461: Cryo-EM structure of jasmonic acid transporter ABCG16 in digitonin 手法: EM (単粒子) / 解像度: 3.18 Å
化合物	ChemComp-JAA: {(1R,2R)-3-oxo-2-[(2Z)-pent-2-en-1-yl]cyclopentyl}acetic acid / ホルモンYM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-VO4: VANADATE ION ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG: Unknown entry ChemComp-HOH: WATER
由来	arabidopsis thaliana (シロイヌナズナ) Arabidopsis (植物)
キーワード	MEMBRANE PROTEIN / jasmonate / transport / cryo-EM / ABC transporter / plant hormone