EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Gigadalton-scale shape-programmable DNA assemblies.
ジャーナル・号・ページ	Nature, Vol. 552, Issue 7683, Page 78-83, Year 2017
掲載日	2017年12月6日
著者	Klaus F Wagenbauer / Christian Sigl / Hendrik Dietz /
PubMed 要旨	Natural biomolecular assemblies such as molecular motors, enzymes, viruses and subcellular structures often form by self-limiting hierarchical oligomerization of multiple subunits. Large structures ...Natural biomolecular assemblies such as molecular motors, enzymes, viruses and subcellular structures often form by self-limiting hierarchical oligomerization of multiple subunits. Large structures can also assemble efficiently from a few components by combining hierarchical assembly and symmetry, a strategy exemplified by viral capsids. De novo protein design and RNA and DNA nanotechnology aim to mimic these capabilities, but the bottom-up construction of artificial structures with the dimensions and complexity of viruses and other subcellular components remains challenging. Here we show that natural assembly principles can be combined with the methods of DNA origami to produce gigadalton-scale structures with controlled sizes. DNA sequence information is used to encode the shapes of individual DNA origami building blocks, and the geometry and details of the interactions between these building blocks then control their copy numbers, positions and orientations within higher-order assemblies. We illustrate this strategy by creating planar rings of up to 350 nanometres in diameter and with atomic masses of up to 330 megadaltons, micrometre-long, thick tubes commensurate in size to some bacilli, and three-dimensional polyhedral assemblies with sizes of up to 1.2 gigadaltons and 450 nanometres in diameter. We achieve efficient assembly, with yields of up to 90 per cent, by using building blocks with validated structure and sufficient rigidity, and an accurate design with interaction motifs that ensure that hierarchical assembly is self-limiting and able to proceed in equilibrium to allow for error correction. We expect that our method, which enables the self-assembly of structures with sizes approaching that of viruses and cellular organelles, can readily be used to create a range of other complex structures with well defined sizes, by exploiting the modularity and high degree of addressability of the DNA origami building blocks used.
リンク	Nature / PubMed:29219966
手法	EM (単粒子)
解像度	21.0 - 31.0 Å
構造データ	EMDB-3826: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: triangular brick for polyhedra 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-3827: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: triangular brick for polyhedra 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-3828: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: triangular brick for polyhedra 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-3829: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: V-brick for the assembly into 2D ring-like objects 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-3830: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: V-brick 2.0 for the assembly into 2D finite-size rings 手法: EM (単粒子) / 解像度: 31.0 Å EMDB-3831: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: reactive vertex for the 3D finite-size assembly into a dodecahedron 手法: EM (単粒子) / 解像度: 31.0 Å EMDB-3832: Shape-programmable self-limiting hierarchical self-assembly of finite-size objects through accurate DNA design: inert vertex for the hierarchical assembly into the reactive vertex object 手法: EM (単粒子) / 解像度: 25.0 Å
由来	synthetic construct (人工物)