EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for sugar perception by gustatory receptors.
ジャーナル・号・ページ	Science, Vol. 383, Issue 6685, Page eadj2609, Year 2024
掲載日	2024年2月23日
著者	Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo /
PubMed 要旨	Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.
リンク	Science / PubMed:38305684
手法	EM (単粒子)
解像度	2.5 - 3.1 Å
構造データ	36410 8jm9 EMDB-36410, PDB-8jm9: The cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster 手法: EM (単粒子) / 解像度: 2.6 Å 36411 8jma EMDB-36411, PDB-8jma: The cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster in complex with fructose 手法: EM (単粒子) / 解像度: 2.5 Å 36417 8jme EMDB-36417, PDB-8jme: The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster 手法: EM (単粒子) / 解像度: 2.5 Å 36421 8jmh EMDB-36421, PDB-8jmh: The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster in complex with sucrose 手法: EM (単粒子) / 解像度: 2.5 Å 36422 8jmi EMDB-36422, PDB-8jmi: The cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster in complex with maltose 手法: EM (単粒子) / 解像度: 2.6 Å 38133 8x82 EMDB-38133, PDB-8x82: The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster 手法: EM (単粒子) / 解像度: 2.8 Å 38134 8x83 EMDB-38134, PDB-8x83: The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose 手法: EM (単粒子) / 解像度: 2.8 Å 38135 8x84 EMDB-38135, PDB-8x84: The cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose and calcium 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-FRU: beta-D-fructofuranose / β-D-フルクトフラノ-ス ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH: WATER ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	drosophila melanogaster (キイロショウジョウバエ)
キーワード	MEMBRANE PROTEIN / gustatory receptor / Gr43a / ligand-gated ion channel / fructose / Gr64a / sucrose / maltose