EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 113, Issue 13, Page E1917-E1926, Year 2016
掲載日	2016年3月29日
著者	Morgan Beeby / Deborah A Ribardo / Caitlin A Brennan / Edward G Ruby / Grant J Jensen / David R Hendrixson /
PubMed 要旨	Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference better, we combined genetic ...Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference better, we combined genetic analyses with electron cryo-tomography subtomogram averaging to determine in situ structures of flagellar motors that produce different torques, from Campylobacter and Vibrio species. For the first time, to our knowledge, our results unambiguously locate the torque-generating stator complexes and show that diverse high-torque motors use variants of an ancestrally related family of structures to scaffold incorporation of additional stator complexes at wider radii from the axial driveshaft than in the model enteric motor. We identify the protein components of these additional scaffold structures and elucidate their sequential assembly, demonstrating that they are required for stator-complex incorporation. These proteins are widespread, suggesting that different bacteria have tailored torques to specific environments by scaffolding alternative stator placement and number. Our results quantitatively account for different motor torques, complete the assignment of the locations of the major flagellar components, and provide crucial constraints for understanding mechanisms of torque generation and the evolution of multiprotein complexes.
リンク	Proc Natl Acad Sci U S A / PubMed:26976588 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	44.0 - 85.3 Å
構造データ	EMDB-3150: Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold 手法: EM (サブトモグラム平均) / 解像度: 44.0 Å EMDB-3154: Salmonella enterica wild-type bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 69.4 Å EMDB-3155: Vibrio fischeri wild-type bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 63.0 Å EMDB-3156: Vibrio fischeri motB deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 70.3 Å EMDB-3157: Campylobacter jejuni motB deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 45.8 Å EMDB-3158: Campylobacter jejuni flgQ deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 60.1 Å EMDB-3159: Campylobacter jejuni flgP deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 61.2 Å EMDB-3160: Campylobacter jejuni pflA deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 85.3 Å EMDB-3161: Campylobacter jejuni pflB deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 61.9 Å EMDB-3162: Vibrio fischeri flgP deletion bacterial flagellar motor 手法: EM (サブトモグラム平均) / 解像度: 81.0 Å EMDB-3813: Tomogram of the salmonella enterica wild-type bacterial flagellar motor 手法: EM (トモグラフィー)
由来	Campylobacter jejuni (カンピロバクター) Salmonella enterica (サルモネラ菌) Aliivibrio fischeri (バクテリア) Salmonella (サルモネラ菌)