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-Structure paper
タイトル | Cryo-EM structures of the human INO80 chromatin-remodeling complex. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 25, Issue 1, Page 37-44, Year 2018 |
掲載日 | 2017年12月4日 |
![]() | Ricardo J Aramayo / Oliver Willhoft / Rafael Ayala / Rohan Bythell-Douglas / Dale B Wigley / Xiaodong Zhang / ![]() |
PubMed 要旨 | Access to chromatin for processes such as transcription and DNA repair requires the sliding of nucleosomes along DNA. This process is aided by chromatin-remodeling complexes, such as the multisubunit ...Access to chromatin for processes such as transcription and DNA repair requires the sliding of nucleosomes along DNA. This process is aided by chromatin-remodeling complexes, such as the multisubunit INO80 chromatin-remodeling complex. Here we present cryo-EM structures of the active core complex of human INO80 at 9.6 Å, with portions at 4.1-Å resolution, and reconstructions of combinations of subunits. Together, these structures reveal the architecture of the INO80 complex, including Ino80 and actin-related proteins, which is assembled around a single RUVBL1 (Tip49a) and RUVBL2 (Tip49b) AAA+ heterohexamer. An unusual spoked-wheel structural domain of the Ino80 subunit is engulfed by this heterohexamer; both, in combination, form the core of the complex. We also identify a cleft in RUVBL1 and RUVBL2, which forms a major interaction site for partner proteins and probably communicates these interactions to its nucleotide-binding sites. |
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手法 | EM (単粒子) |
解像度 | 4.06 - 11.5 Å |
構造データ | ![]() EMDB-3772: EMDB-3773: High resolution INO80 core complex ![]() EMDB-3774: ![]() EMDB-3775: |
化合物 | ![]() ChemComp-ADP: |
由来 |
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![]() | GENE REGULATION / Chromatin remodelling complex |