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-Structure paper
タイトル | The Complete Structure of the Mycobacterium smegmatis 70S Ribosome. |
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ジャーナル・号・ページ | Cell Rep, Vol. 20, Issue 1, Page 149-160, Year 2017 |
掲載日 | 2017年7月5日 |
著者 | Jendrik Hentschel / Chloe Burnside / Ingrid Mignot / Marc Leibundgut / Daniel Boehringer / Nenad Ban / |
PubMed 要旨 | The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death ...The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. |
リンク | Cell Rep / PubMed:28683309 |
手法 | EM (単粒子) |
解像度 | 3.18 - 3.45 Å |
構造データ | EMDB-3748: Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-PHE: |
由来 |
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キーワード | RIBOSOME / translation |