+検索条件
-Structure paper
タイトル | Structure-guided discovery of protein and glycan components in native mastigonemes. |
---|---|
ジャーナル・号・ページ | Cell, Vol. 187, Issue 7, Page 1733-1744.e12, Year 2024 |
掲載日 | 2024年3月28日 |
著者 | Junhao Huang / Hui Tao / Jikun Chen / Yang Shen / Jianlin Lei / Junmin Pan / Chuangye Yan / Nieng Yan / |
PubMed 要旨 | Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we ...Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we report the cryo-EM structure of native mastigonemes isolated from Chlamydomonas at 3.0 Å resolution. The long stem assembles as a super spiral, with each helical turn comprising four pairs of anti-parallel mastigoneme-like protein 1 (Mst1). A large array of arabinoglycans, which represents a common class of glycosylation in plants and algae, is resolved surrounding the type II poly-hydroxyproline (Hyp) helix in Mst1. The EM map unveils a mastigoneme axial protein (Mstax) that is rich in heavily glycosylated Hyp and contains a PKD2-like transmembrane domain (TMD). Mstax, with nearly 8,000 residues spanning from the intracellular region to the distal end of the mastigoneme, provides the framework for Mst1 assembly. Our study provides insights into the complexity of protein and glycan interactions in native bio-architectures. |
リンク | Cell / PubMed:38552612 |
手法 | EM (単粒子) |
解像度 | 3.0 Å |
構造データ | EMDB-37389, PDB-8wa2: |
化合物 | ChemComp-CA: ChemComp-AHR: ChemComp-GLA: ChemComp-NAG: |
由来 |
|
キーワード | MEMBRANE PROTEIN / Cilia / arabinoglycan / PKD2 / PPII helix / mechanosensation. |