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Yorodumi- PDB-8wa2: cryo-EM structure of native mastigonemes isolated from Chlamydomo... -
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-Basic information
Entry | Database: PDB / ID: 8wa2 | ||||||
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Title | cryo-EM structure of native mastigonemes isolated from Chlamydomonas reinhardtii at 3.0 angstrom resolution | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / Cilia / arabinoglycan / PKD2 / PPII helix / mechanosensation. | ||||||
Function / homology | Tyrosine-protein kinase ephrin type A/B receptor-like / Tyrosine-protein kinase ephrin type A/B receptor-like / Putative ephrin-receptor like / Growth factor receptor cysteine-rich domain superfamily / alpha-L-arabinofuranose / alpha-D-galactopyranose / Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) synthetic construct (others) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | ||||||
Authors | Huang, J. / Tao, H. / Chen, J. / Pan, J. / Yan, C. / Yan, N. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell / Year: 2024 Title: Structure-guided discovery of protein and glycan components in native mastigonemes. Authors: Junhao Huang / Hui Tao / Jikun Chen / Yang Shen / Jianlin Lei / Junmin Pan / Chuangye Yan / Nieng Yan / Abstract: Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we ...Mastigonemes, the hair-like lateral appendages lining cilia or flagella, participate in mechanosensation and cellular motion, but their constituents and structure have remained unclear. Here, we report the cryo-EM structure of native mastigonemes isolated from Chlamydomonas at 3.0 Å resolution. The long stem assembles as a super spiral, with each helical turn comprising four pairs of anti-parallel mastigoneme-like protein 1 (Mst1). A large array of arabinoglycans, which represents a common class of glycosylation in plants and algae, is resolved surrounding the type II poly-hydroxyproline (Hyp) helix in Mst1. The EM map unveils a mastigoneme axial protein (Mstax) that is rich in heavily glycosylated Hyp and contains a PKD2-like transmembrane domain (TMD). Mstax, with nearly 8,000 residues spanning from the intracellular region to the distal end of the mastigoneme, provides the framework for Mst1 assembly. Our study provides insights into the complexity of protein and glycan interactions in native bio-architectures. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wa2.cif.gz | 2.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8wa2.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8wa2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8wa2_validation.pdf.gz | 9.8 MB | Display | wwPDB validaton report |
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Full document | 8wa2_full_validation.pdf.gz | 10.3 MB | Display | |
Data in XML | 8wa2_validation.xml.gz | 290.9 KB | Display | |
Data in CIF | 8wa2_validation.cif.gz | 448.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wa/8wa2 ftp://data.pdbj.org/pub/pdb/validation_reports/wa/8wa2 | HTTPS FTP |
-Related structure data
Related structure data | 37389MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein , 2 types, 9 molecules ABCDEFGHI
#1: Protein | Mass: 206316.203 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J9H7 #2: Protein | Mass: 6239.464 Da / Num. of mol.: 3 / Source method: obtained synthetically Details: Our Mstax sequence in the structure (chain G, H and I) represents the average sequence resulting from the statistical analysis of a segment of 34 repeated sequences in Mstax. Source: (synth.) synthetic construct (others) |
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