+検索条件
-Structure paper
タイトル | A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 3187, Year 2024 |
掲載日 | 2024年4月15日 |
著者 | Jing Zhang / Shuo Zhang / Wei Zhou / Xiang Zhang / Guanjin Li / Ruoxuan Li / Xingyu Lin / Ziying Chen / Fang Liu / Pan Shen / Xiaogen Zhou / Yue Gao / Zhenguo Chen / Yanjie Chao / Chengyuan Wang / |
PubMed 要旨 | Transcription is crucial for the expression of genetic information and its efficient and accurate termination is required for all living organisms. Rho-dependent termination could rapidly terminate ...Transcription is crucial for the expression of genetic information and its efficient and accurate termination is required for all living organisms. Rho-dependent termination could rapidly terminate unwanted premature RNAs and play important roles in bacterial adaptation to changing environments. Although Rho has been discovered for about five decades, the regulation mechanisms of Rho-dependent termination are still not fully elucidated. Here we report that Rof is a conserved antiterminator and determine the cryogenic electron microscopy structure of Rho-Rof antitermination complex. Rof binds to the open-ring Rho hexamer and inhibits the initiation of Rho-dependent termination. Rof's N-terminal α-helix undergoes conformational changes upon binding with Rho, and is key in facilitating Rof-Rho interactions. Rof binds to Rho's primary binding site (PBS) and excludes Rho from binding with PBS ligand RNA at the initiation step. Further in vivo analyses in Salmonella Typhimurium show that Rof is required for virulence gene expression and host cell invasion, unveiling a physiological function of Rof and transcription termination in bacterial pathogenesis. |
リンク | Nat Commun / PubMed:38622116 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 Å |
構造データ | EMDB-37342, PDB-8w8d: |
化合物 | ChemComp-HOH: |
由来 |
|
キーワード | TRANSCRIPTION (転写 (生物学)) / Escherichia coli Rho-Rof complex / Rho-dependent termination / antitermination |