EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Overcoming resolution attenuation during tilted cryo-EM data collection.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 389, Year 2024
掲載日	2024年1月9日
著者	Sriram Aiyer / Philip R Baldwin / Shi Min Tan / Zelin Shan / Juntaek Oh / Atousa Mehrani / Marianne E Bowman / Gordon Louie / Dario Oliveira Passos / Selena Đorđević-Marquardt / Mario Mietzsch / Joshua A Hull / Shuichi Hoshika / Benjamin A Barad / Danielle A Grotjahn / Robert McKenna / Mavis Agbandje-McKenna / Steven A Benner / Joseph A P Noel / Dong Wang / Yong Zi Tan / Dmitry Lyumkis /
PubMed 要旨	Structural biology efforts using cryogenic electron microscopy are frequently stifled by specimens adopting "preferred orientations" on grids, leading to anisotropic map resolution and impeding ...Structural biology efforts using cryogenic electron microscopy are frequently stifled by specimens adopting "preferred orientations" on grids, leading to anisotropic map resolution and impeding structure determination. Tilting the specimen stage during data collection is a generalizable solution but has historically led to substantial resolution attenuation. Here, we develop updated data collection and image processing workflows and demonstrate, using multiple specimens, that resolution attenuation is negligible or significantly reduced across tilt angles. Reconstructions with and without the stage tilted as high as 60° are virtually indistinguishable. These strategies allowed the reconstruction to 3 Å resolution of a bacterial RNA polymerase with preferred orientation, containing an unnatural nucleotide for studying novel base pair recognition. Furthermore, we present a quantitative framework that allows cryo-EM practitioners to define an optimal tilt angle during data acquisition. These results reinforce the utility of employing stage tilt for data collection and provide quantitative metrics to obtain isotropic maps.
リンク	Nat Commun / PubMed:38195598 / PubMed Central
手法	EM (単粒子)
解像度	1.92 - 3.2 Å
構造データ	EMDB-36766: Untilted (0 Degree Tilted) Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36767: 10 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36768: 20 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36769: 30 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36770: 40 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36771: 50 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-36772: 60 Degree Tilted Single-Particle CryoEM Reconstruction of AAV2 Viral Capsid 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-36807: Untilted (0 Degree Tilted) Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-36809: 10 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-36810: 20 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-36811: 30 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-36812: 40 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-36813: 60 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-36814: 50 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-36816: Untilted (0 Degree Tilted) Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-36817: 10 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-36818: 20 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-36819: 30 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-36820: 40 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-36821: 50 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-36822: 60 Degree Tilted Single-Particle CryoEM Reconstruction of DPS 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-41230: Untilted (0 Degree Tilted) Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 1.92 Å EMDB-41231: 30 Degree Tilted Single-Particle CryoEM Reconstruction of Apoferritin 手法: EM (単粒子) / 解像度: 1.99 Å 41695 8txo EMDB-41695: 60 Degree Tilted Single-Particle CryoEM Reconstruction of RNA polymerase PDB-8txo: E. coli DNA-directed RNA polymerase transcription elongation complex bound to the unnatural dZ-PTP base pair in the active site 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-S9F: [[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-1$l^{4},3,5,7-tetrazabicyclo[4.3.0]nona-1(6),3,8-trien-7-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate
由来	Homo sapiens (ヒト) escherichia coli (大腸菌) synthetic construct (人工物)
キーワード	TRANSCRIPTION / complex / unnatural base pairs / synthetic biology