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-Structure paper
タイトル | RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 45, Issue 11, Page 6945-6959, Year 2017 |
掲載日 | 2017年6月20日 |
著者 | Jorge Pedro López-Alonso / Tatsuya Kaminishi / Takeshi Kikuchi / Yuya Hirata / Idoia Iturrioz / Neha Dhimole / Andreas Schedlbauer / Yoichi Hase / Simon Goto / Daisuke Kurita / Akira Muto / Shu Zhou / Chieko Naoe / Deryck J Mills / David Gil-Carton / Chie Takemoto / Hyouta Himeno / Paola Fucini / Sean R Connell / |
PubMed 要旨 | During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, ...During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis. |
リンク | Nucleic Acids Res / PubMed:28482099 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 5.16 Å |
構造データ | EMDB-3661, PDB-5no2: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-GNP: |
由来 |
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キーワード | RIBOSOME |