EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in egress.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 114, Issue 13, Page 3439-3444, Year 2017
掲載日	2017年3月28日
著者	Victoria L Hale / Jean M Watermeyer / Fiona Hackett / Gema Vizcay-Barrena / Christiaan van Ooij / James A Thomas / Matthew C Spink / Maria Harkiolaki / Elizabeth Duke / Roland A Fleck / Michael J Blackman / Helen R Saibil /
PubMed 要旨	In the asexual blood stages of malarial infection, merozoites invade erythrocytes and replicate within a parasitophorous vacuole to form daughter cells that eventually exit (egress) by sequential ...In the asexual blood stages of malarial infection, merozoites invade erythrocytes and replicate within a parasitophorous vacuole to form daughter cells that eventually exit (egress) by sequential rupture of the vacuole and erythrocyte membranes. The current model is that PKG, a malarial cGMP-dependent protein kinase, triggers egress, activating malarial proteases and other effectors. Using selective inhibitors of either PKG or cysteine proteases to separately inhibit the sequential steps in membrane perforation, combined with video microscopy, electron tomography, electron energy loss spectroscopy, and soft X-ray tomography of mature intracellular parasites, we resolve intermediate steps in egress. We show that the parasitophorous vacuole membrane (PVM) is permeabilized 10-30 min before its PKG-triggered breakdown into multilayered vesicles. Just before PVM breakdown, the host red cell undergoes an abrupt, dramatic shape change due to the sudden breakdown of the erythrocyte cytoskeleton, before permeabilization and eventual rupture of the erythrocyte membrane to release the parasites. In contrast to the previous view of PKG-triggered initiation of egress and a gradual dismantling of the host erythrocyte cytoskeleton over the course of schizont development, our findings identify an initial step in egress and show that host cell cytoskeleton breakdown is restricted to a narrow time window within the final stages of egress.
リンク	Proc Natl Acad Sci U S A / PubMed:28292906 / PubMed Central
手法	EM (トモグラフィー)
構造データ	EMDB-3586: Malaria-infected red blood cell section showing partially segmented schizont 手法: EM (トモグラフィー) EMDB-3587: Malaria infected red blood cell section showing a schizont at an intermediate stage in parasite egress 手法: EM (トモグラフィー) EMDB-3606: Malaria-infected red blood cell section showing a segmented schizont 手法: EM (トモグラフィー) EMDB-3610: Malaria-infected red blood cell section showing schizont stalled in egress with the inhibitor E64 手法: EM (トモグラフィー)
由来	Plasmodium falciparum 3D7 (マラリア病原虫)