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-Structure paper
タイトル | DNA looping mediates cooperative transcription activation. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 31, Issue 2, Page 293-299, Year 2024 |
掲載日 | 2024年1月4日 |
著者 | Shu-Jing Han / Yong-Liang Jiang / Lin-Lin You / Li-Qiang Shen / Xiaoxian Wu / Feng Yang / Ning Cui / Wen-Wen Kong / Hui Sun / Ke Zhou / Hui-Chao Meng / Zhi-Peng Chen / Yuxing Chen / Yu Zhang / Cong-Zhao Zhou / |
PubMed 要旨 | Transcription factors respond to multilevel stimuli and co-occupy promoter regions of target genes to activate RNA polymerase (RNAP) in a cooperative manner. To decipher the molecular mechanism, here ...Transcription factors respond to multilevel stimuli and co-occupy promoter regions of target genes to activate RNA polymerase (RNAP) in a cooperative manner. To decipher the molecular mechanism, here we report two cryo-electron microscopy structures of Anabaena transcription activation complexes (TACs): NtcA-TAC composed of RNAP holoenzyme, promoter and a global activator NtcA, and NtcA-NtcB-TAC comprising an extra context-specific regulator, NtcB. Structural analysis showed that NtcA binding makes the promoter DNA bend by ∼50°, which facilitates RNAP to contact NtcB at the distal upstream NtcB box. The sequential binding of NtcA and NtcB induces looping back of promoter DNA towards RNAP, enabling the assembly of a fully activated TAC bound with two activators. Together with biochemical assays, we propose a 'DNA looping' mechanism of cooperative transcription activation in bacteria. |
リンク | Nat Struct Mol Biol / PubMed:38177666 |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.35 - 7.6 Å |
構造データ | EMDB-34475, PDB-8h3v: EMDB-34476, PDB-8h40: EMDB-34477: Cryo-EM structure of the full transcription activation complex NtcA-NtcB-TAC focusing on NtcA and NtcB binding sites PDB-8h3z: |
化合物 | ChemComp-IOD: ChemComp-HOH: |
由来 |
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キーワード | TRANSCRIPTION (転写 (生物学)) / transcription activation complex / coordinated regulation / TRANSCRIPTION/DNA / LysR-type transcription factor / TRANSCRIPTION-DNA complex |