EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 3537, Year 2023
掲載日	2023年6月15日
著者	Yu Guo / Guangshun Zhang / Qi Yang / Xiaowei Xie / Yang Lu / Xuelian Cheng / Hui Wang / Jingxi Liang / Jielin Tang / Yuxin Gao / Hang Shang / Jun Dai / Yongxia Shi / Jiaxi Zhou / Jun Zhou / Hangtian Guo / Haitao Yang / Jianwei Qi / Lijun Liu / Shihui Ma / Biao Zhang / Qianyu Huo / Yi Xie / Junping Wu / Fang Dong / Song Zhang / Zhiyong Lou / Yan Gao / Zidan Song / Wenming Wang / Zixian Sun / Xiaoming Yang / Dongsheng Xiong / Fengjiang Liu / Xinwen Chen / Ping Zhu / Ximo Wang / Tao Cheng / Zihe Rao /
PubMed 要旨	The SARS-CoV-2 Omicron variant evades most currently approved neutralizing antibodies (nAbs) and caused drastic decrease of plasma neutralizing activity elicited by vaccination or prior infection, ...The SARS-CoV-2 Omicron variant evades most currently approved neutralizing antibodies (nAbs) and caused drastic decrease of plasma neutralizing activity elicited by vaccination or prior infection, urging the need for the development of pan-variant antivirals. Breakthrough infection induces a hybrid immunological response with potentially broad, potent and durable protection against variants, therefore, convalescent plasma from breakthrough infection may provide a broadened repertoire for identifying elite nAbs. We performed single-cell RNA sequencing (scRNA-seq) and BCR sequencing (scBCR-seq) of B cells from BA.1 breakthrough-infected patients who received 2 or 3 previous doses of inactivated vaccine. Elite nAbs, mainly derived from the IGHV2-5 and IGHV3-66/53 germlines, showed potent neutralizing activity across Wuhan-Hu-1, Delta, Omicron sublineages BA.1 and BA.2 at picomolar NT values. Cryo-EM analysis revealed diverse modes of spike recognition and guides the design of cocktail therapy. A single injection of paired antibodies cocktail provided potent protection in the K18-hACE2 transgenic female mouse model of SARS-CoV-2 infection.
リンク	Nat Commun / PubMed:37322000 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.51 - 3.8 Å
構造データ	34124 7yve EMDB-34124, PDB-7yve: Omicron BA.4/5 SARS-CoV-2 S in complex with TH027 Fab 手法: EM (単粒子) / 解像度: 3.4 Å 34125 7yvf EMDB-34125, PDB-7yvf: Omicron BA.4/5 SARS-CoV-2 S RBD in complex with TH027 Fab 手法: EM (単粒子) / 解像度: 3.4 Å 34126 7yvg EMDB-34126, PDB-7yvg: Omicron BA.4/5 SARS-CoV-2 S in complex with TH132 Fab 手法: EM (単粒子) / 解像度: 3.4 Å 34127 7yvh EMDB-34127, PDB-7yvh: Omicron BA.4/5 SARS-CoV-2 S RBD in complex with TH132 Fab 手法: EM (単粒子) / 解像度: 3.6 Å 34128 7yvi EMDB-34128, PDB-7yvi: Omicron BA.4/5 SARS-CoV-2 S in complex with TH236 Fab 手法: EM (単粒子) / 解像度: 3.7 Å 34129 7yvj EMDB-34129, PDB-7yvj: Omicron BA.4/5 SARS-CoV-2 S RBD in complex with TH236 Fab 手法: EM (単粒子) / 解像度: 3.6 Å 34130 7yvk EMDB-34130, PDB-7yvk: Omicron BA.4/5 SARS-CoV-2 S in complex with TH272 Fab 手法: EM (単粒子) / 解像度: 3.2 Å 34131 7yvl EMDB-34131, PDB-7yvl: Omicron BA.4/5 SARS-CoV-2 S RBD in complex with TH272 Fab 手法: EM (単粒子) / 解像度: 3.3 Å 34132 7yvm EMDB-34132, PDB-7yvm: Omicron BA.4/5 SARS-CoV-2 S RBD in complex with TH272 Fab 手法: EM (単粒子) / 解像度: 3.5 Å 34133 7yvn EMDB-34133, PDB-7yvn: Omicron BA.4/5 SARS-CoV-2 S in complex with TH281 Fab 手法: EM (単粒子) / 解像度: 3.4 Å 34134 7yvo EMDB-34134, PDB-7yvo: Omicron BA.4/5 SARS-CoV-2 S in complex with TH027/132 Fab 手法: EM (単粒子) / 解像度: 3.3 Å 34135 7yvp EMDB-34135, PDB-7yvp: Omicron BA.4/5 SARS-CoV-2 S in complex with TH272/281 Fab 手法: EM (単粒子) / 解像度: 3.8 Å 34181 8gou EMDB-34181, PDB-8gou: Omicron BA.4/5 SARS-CoV-2 S in complex with TH003 Fab 手法: EM (単粒子) / 解像度: 3.7 Å PDB-8gpy: Crystal structure of Omicron BA.4/5 RBD in complex with a neutralizing antibody scFv 手法: X-RAY DIFFRACTION / 解像度: 2.51 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-HOH: WATER / 水
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/ANTIVIRAL PROTEIN (ウイルス性) / COVID-19 (新型コロナウイルス感染症 (2019年)) / spike glycoprotein (スパイクタンパク質) / virus (ウイルス) / VIRAL PROTEIN (ウイルスタンパク質) / VIRAL PROTEIN-ANTIVIRAL PROTEIN complex (ウイルス性) / ANTIVIRAL PROTEIN/VIRAL PROTEIN (抗ウイルス薬) / ANTIVIRAL PROTEIN-VIRAL PROTEIN complex (抗ウイルス薬) / Omicron (Ο) / crystal strcture / neutralizing antibody (中和抗体) / scFV (単鎖可変領域フラグメント)