EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 7549, Year 2022
掲載日	2022年12月7日
著者	Ning Cui / Jun-Tao Zhang / Zhuolin Li / Xiao-Yu Liu / Chongyuan Wang / Hongda Huang / Ning Jia /
PubMed 要旨	The RNA-targeting type III-E CRISPR-gRAMP effector interacts with a caspase-like protease TPR-CHAT to form the CRISPR-guided caspase complex (Craspase), but their functional mechanism is unknown. ...The RNA-targeting type III-E CRISPR-gRAMP effector interacts with a caspase-like protease TPR-CHAT to form the CRISPR-guided caspase complex (Craspase), but their functional mechanism is unknown. Here, we report cryo-EM structures of the type III-E gRAMP and gRAMP-TPR-CHAT complexes, before and after either self or non-self RNA target binding, and elucidate the mechanisms underlying RNA-targeting and non-self RNA-induced protease activation. The associated TPR-CHAT adopted a distinct conformation upon self versus non-self RNA target binding, with nucleotides at positions -1 and -2 of the CRISPR-derived RNA (crRNA) serving as a sensor. Only binding of the non-self RNA target activated the TPR-CHAT protease, leading to cleavage of Csx30 protein. Furthermore, TPR-CHAT structurally resembled eukaryotic separase, but with a distinct mechanism for protease regulation. Our findings should facilitate the development of gRAMP-based RNA manipulation tools, and advance our understanding of the virus-host discrimination process governed by a nuclease-protease Craspase during type III-E CRISPR-Cas immunity.
リンク	Nat Commun / PubMed:36477448 / PubMed Central
手法	EM (単粒子)
解像度	2.54 - 2.93 Å
構造データ	33676 7y80 EMDB-33676: CryoEM structure of type III-E CRISPR gRAMP-crRNA binary complex PDB-7y80: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA binary complex 手法: EM (単粒子) / 解像度: 2.71 Å 33677 7y81 EMDB-33677: CryoEM structure of type III-E CRISPR gRAMP-crRNA complex bound to non-self RNA target PDB-7y81: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA complex bound to non-self RNA target 手法: EM (単粒子) / 解像度: 2.54 Å 33678 7y82 EMDB-33678: CryoEM structure of type III-E CRISPR gRAMP-crRNA complex bound to self RNA target PDB-7y82: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA complex bound to self RNA target 手法: EM (単粒子) / 解像度: 2.83 Å 33679 7y83 EMDB-33679: CryoEM structure of type III-E CRISPR gRAMP-crRNA in complex with TPR-CHAT bound to non-self RNA target PDB-7y83: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA in complex with TPR-CHAT protease bound to non-self RNA target 手法: EM (単粒子) / 解像度: 2.93 Å 33680 7y84 EMDB-33680: CryoEM structure of type III-E CRISPR gRAMP-crRNA in complex with TPR-CHAT PDB-7y84: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA in complex with TPR-CHAT protease 手法: EM (単粒子) / 解像度: 2.61 Å 33681 7y85 EMDB-33681: CryoEM structure of type III-E CRISPR gRAMP-crRNA in complex with TPR-CHAT bound to self RNA target PDB-7y85: CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA in complex with TPR-CHAT protease bound to self RNA target 手法: EM (単粒子) / 解像度: 2.73 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	candidatus scalindua brodae (バクテリア)
キーワード	STRUCTURAL PROTEIN/RNA / Nuclease / STRUCTURAL PROTEIN-RNA COMPLEX / RNA BINDING PROTEIN