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-Structure paper
タイトル | Structure of promoter-bound TFIID and model of human pre-initiation complex assembly. |
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ジャーナル・号・ページ | Nature, Vol. 531, Issue 7596, Page 604-609, Year 2016 |
掲載日 | 2016年3月31日 |
著者 | Robert K Louder / Yuan He / José Ramón López-Blanco / Jie Fang / Pablo Chacón / Eva Nogales / |
PubMed 要旨 | The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the ...The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation. |
リンク | Nature / PubMed:27007846 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 7.2 - 10.2 Å |
構造データ | EMDB-3304: EMDB-3305: Structure of human TFIID-IIA bound to core promoter DNA (locally-refined BC core) EMDB-3306: EMDB-3307: |
由来 |
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キーワード | TRANSCRIPTION / TFIID / TFIIA / RNA POLYMERASE II / GENERAL TRANSCRIPTION FACTORS / PREINITIATION COMPLEX / CORE PROMOTER / DNA BINDING |