+検索条件
-Structure paper
タイトル | Structure of the ISW1a complex bound to the dinucleosome. |
---|---|
ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 31, Issue 2, Page 266-274, Year 2024 |
掲載日 | 2024年1月4日 |
著者 | Lifei Li / Kangjing Chen / Youyang Sia / Pengjing Hu / Youpi Ye / Zhucheng Chen / |
PubMed 要旨 | Nucleosomes are basic repeating units of chromatin and form regularly spaced arrays in cells. Chromatin remodelers alter the positions of nucleosomes and are vital in regulating chromatin ...Nucleosomes are basic repeating units of chromatin and form regularly spaced arrays in cells. Chromatin remodelers alter the positions of nucleosomes and are vital in regulating chromatin organization and gene expression. Here we report the cryo-EM structure of chromatin remodeler ISW1a complex from Saccharomyces cerevisiae bound to the dinucleosome. Each subunit of the complex recognizes a different nucleosome. The motor subunit binds to the mobile nucleosome and recognizes the acidic patch through two arginine residues, while the DNA-binding module interacts with the entry DNA at the nucleosome edge. This nucleosome-binding mode provides the structural basis for linker DNA sensing of the motor. Notably, the Ioc3 subunit recognizes the disk face of the adjacent nucleosome through interacting with the H4 tail, the acidic patch and the nucleosomal DNA, which plays a role in the spacing activity in vitro and in nucleosome organization and cell fitness in vivo. Together, these findings support the nucleosome spacing activity of ISW1a and add a new mode of nucleosome remodeling in the context of a chromatin environment. |
リンク | Nat Struct Mol Biol / PubMed:38177688 |
手法 | EM (単粒子) |
解像度 | 3.09 - 5.4 Å |
構造データ | EMDB-32992, PDB-7x3t: EMDB-32994, PDB-7x3v: EMDB-32995, PDB-7x3w: EMDB-32996, PDB-7x3x: |
化合物 | ChemComp-ADP: ChemComp-BEF: ChemComp-MG: |
由来 |
|
キーワード | PROTEIN BINDING / Ioc3 binding / PROTEIN BINDING/DNA / PROTEIN BINDING-DNA COMPLEX |