EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and function of H/K pump mutants reveal Na/K pump mechanisms.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 5270, Year 2022
掲載日	2022年9月9日
著者	Victoria C Young / Hanayo Nakanishi / Dylan J Meyer / Tomohiro Nishizawa / Atsunori Oshima / Pablo Artigas / Kazuhiro Abe /
PubMed 要旨	Ion-transport mechanisms evolve by changing ion-selectivity, such as switching from Na to H selectivity in secondary-active transporters or P-type-ATPases. Here we study primary-active transport via ...Ion-transport mechanisms evolve by changing ion-selectivity, such as switching from Na to H selectivity in secondary-active transporters or P-type-ATPases. Here we study primary-active transport via P-type ATPases using functional and structural analyses to demonstrate that four simultaneous residue substitutions transform the non-gastric H/K pump, a strict H-dependent electroneutral P-type ATPase, into a bona fide Na-dependent electrogenic Na/K pump. Conversion of a H-dependent primary-active transporter into a Na-dependent one provides a prototype for similar studies of ion-transport proteins. Moreover, we solve the structures of the wild-type non-gastric H/K pump, a suitable drug target to treat cystic fibrosis, and of its Na/K pump-mimicking mutant in two major conformations, providing insight on how Na binding drives a concerted mechanism leading to Na/K pump phosphorylation.
リンク	Nat Commun / PubMed:36085139 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.8 - 3.4 Å
構造データ	32954 7x21 EMDB-32954, PDB-7x21: Cryo-EM structure of non gastric H,K-ATPase alpha2 K794A in (K+)E2-AlF state 手法: EM (単粒子) / 解像度: 2.8 Å 32955 7x22 EMDB-32955, PDB-7x22: Cryo-EM structure of non gastric H,K-ATPase alpha2 K794S in (2K+)E2-AlF state 手法: EM (単粒子) / 解像度: 3.0 Å 32956 7x23 EMDB-32956, PDB-7x23: Cryo-EM structure of non gastric H,K-ATPase alpha2 SPWC mutant in 3Na+E1-AMPPCPF state 手法: EM (単粒子) / 解像度: 3.2 Å 32957 7x24 EMDB-32957, PDB-7x24: Cryo-EM structure of non gastric H,K-ATPase alpha2 SPWC mutant in (2K+)E2-AlF state 手法: EM (単粒子) / 解像度: 3.4 Å PDB-7x20: Crystal structure of non gastric H,K-ATPase alpha2 in (K+)E2-AlF state 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å
化合物	ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-K: Unknown entry / カリウムカチオン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-PCW: 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / O-(1-O,2-O-ジオレオイル-L-グリセロ-3-ホスホ)コリン / DOPC, リン脂質YM ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-NA: Unknown entry / ナトリウムカチオン
由来	rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE PROTEIN / P-type ATPase / transporter / proton pump / colon / kidney / airway