EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of Omicron spike complexes and implications for neutralizing antibody development.
ジャーナル・号・ページ	Cell Rep, Vol. 39, Issue 5, Page 110770, Year 2022
掲載日	2022年5月3日
著者	Hangtian Guo / Yan Gao / Tinghan Li / Tingting Li / Yuchi Lu / Le Zheng / Yue Liu / Tingting Yang / Feiyang Luo / Shuyi Song / Wei Wang / Xiuna Yang / Henry C Nguyen / Hongkai Zhang / Ailong Huang / Aishun Jin / Haitao Yang / Zihe Rao / Xiaoyun Ji /
PubMed 要旨	The emergence of the SARS-CoV-2 Omicron variant is dominant in many countries worldwide. The high number of spike mutations is responsible for the broad immune evasion from existing vaccines and ...The emergence of the SARS-CoV-2 Omicron variant is dominant in many countries worldwide. The high number of spike mutations is responsible for the broad immune evasion from existing vaccines and antibody drugs. To understand this, we first present the cryo-electron microscopy structure of ACE2-bound SARS-CoV-2 Omicron spike. Comparison to previous spike antibody structures explains how Omicron escapes these therapeutics. Secondly, we report structures of Omicron, Delta, and wild-type spikes bound to a patient-derived Fab antibody fragment (510A5), which provides direct evidence where antibody binding is greatly attenuated by the Omicron mutations, freeing spike to bind ACE2. Together with biochemical binding and 510A5 neutralization assays, our work establishes principles of binding required for neutralization and clearly illustrates how the mutations lead to antibody evasion yet retain strong ACE2 interactions. Structural information on spike with both bound and unbound antibodies collectively elucidates potential strategies for generation of therapeutic antibodies.
リンク	Cell Rep / PubMed:35477022 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.8 Å
構造データ	32739 7ws0 EMDB-32739, PDB-7ws0: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.2 Å 32740 7ws1 EMDB-32740, PDB-7ws1: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.4 Å 32741 7ws2 EMDB-32741, PDB-7ws2: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.3 Å 32742 7ws3 EMDB-32742, PDB-7ws3: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.6 Å 32743 7ws4 EMDB-32743, PDB-7ws4: Ultrapotent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants 手法: EM (単粒子) / 解像度: 3.7 Å 32744 7ws5 EMDB-32744, PDB-7ws5: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.7 Å 32748 7ws6 EMDB-32748, PDB-7ws6: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.8 Å 32749 7ws7 EMDB-32749, PDB-7ws7: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.4 Å 32750 7ws8 EMDB-32750, PDB-7ws8: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.0 Å 32751 7ws9 EMDB-32751, PDB-7ws9: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 2.9 Å 32752 7wsa EMDB-32752, PDB-7wsa: Structures of Omicron Spike complexes illuminate broad-spectrum neutralizing antibody development 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM / COVID-19 / spike glycoprotein / virus / VIRAL PROTEIN / antibody / VIRAL PROTEIN-IMMUNE SYSTEM complex / VIRAL PROTEIN/HYDROLASE / VIRAL PROTEIN-HYDROLASE complex