EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Addressing compressive deformation of proteins embedded in crystalline ice.
ジャーナル・号・ページ	Structure, Vol. 31, Issue 2, Page 213-220.e3, Year 2023
掲載日	2023年2月2日
著者	Huigang Shi / Chunling Wu / Xinzheng Zhang /
PubMed 要旨	For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. ...For cryoelectron microscopy (cryo-EM), high cooling rates have been required for preparation of protein samples to vitrify the surrounding water and avoid formation of damaging crystalline ice. Whether and how crystalline ice affects single-particle cryo-EM is still unclear. Here, single-particle cryo-EM was used to analyze three-dimensional structures of various proteins and viruses embedded in crystalline ice formed at various cooling rates. Low cooling rates led to shrinkage deformation and density distortions on samples having loose structures. Higher cooling rates reduced deformations. Deformation-free proteins in crystalline ice were obtained by modifying the freezing conditions, and reconstructions from these samples revealed a marked improvement over vitreous ice. This procedure also increased the efficiency of cryo-EM structure determinations and was essential for high-resolution reconstructions.
リンク	Structure / PubMed:36586403
手法	EM (単粒子)
解像度	1.76 - 3.2 Å
構造データ	28627 8bqn EMDB-28627: Cryo-EM structure of Coxsackievirus A10 (empty) embedded in crystalline ice PDB-8bqn: Structure of empty Coxsackievirus A10 embedded in crystalline ice frozen at -140 degree 手法: EM (単粒子) / 解像度: 3.1 Å 28638 8f7y EMDB-28638: Cryo-EM structure of Coxsackievirus A10 embedded in crystalline ice PDB-8f7y: Structure of Coxsackievirus A10 frozen at -183 degree embedded in crystalline ice 手法: EM (単粒子) / 解像度: 2.8 Å 28639 8ew0 EMDB-28639, PDB-8ew0: Cryo-EM structure of glutamate dehydrogenase frozen at various temperature 手法: EM (単粒子) / 解像度: 2.7 Å 28640 8ew2 EMDB-28640, PDB-8ew2: Cryo-EM structure of Aldolase embedded in crystalline ice 手法: EM (単粒子) / 解像度: 3.1 Å 32695 8f49 EMDB-32695: High resolution of apo-ferritin embedded in crystalline ice PDB-8f49: 1.8 angstrom structure of apoferritin embedded in crystalline ice 手法: EM (単粒子) / 解像度: 1.76 Å 32703 8hhs EMDB-32703: apo-ferritin embedded in crystalline ice frozen at -183 degree PDB-8hhs: Structure of human apoferritin embedded in crystalline ice 手法: EM (単粒子) / 解像度: 2.4 Å 32709 8hi2 EMDB-32709: EV71 VLP maps embedded in crystalline ice PDB-8hi2: Structure of EV71 VLP frozen at -183 degree embedded in crystalline ice 手法: EM (単粒子) / 解像度: 3.2 Å
由来	bos taurus (ウシ) oryctolagus cuniculus (ウサギ) homo sapiens (ヒト) coxsackievirus a10 (コクサッキーウイルス) enterovirus a71 (エンテロウイルス)
キーワード	VIRUS / Coxsackievirus A10 / empty Coxsackievirus A10 / crystalline ice / LYASE / glutamate dehydrogenase / GDH / Aldolase / METAL BINDING PROTEIN / ferritin / crystal ice / crystal-ice / apo-ferritin / human apo-ferritin / human ferritin / Coxsackievirus