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-Structure paper
タイトル | Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex. |
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ジャーナル・号・ページ | Cell Res, Vol. 32, Issue 5, Page 437-450, Year 2022 |
掲載日 | 2022年3月18日 |
著者 | Zongqiang Li / Shuaijiabin Chen / Liang Zhao / Guoqiang Huang / Xiong Pi / Shan Sun / Peiyi Wang / Sen-Fang Sui / |
PubMed 要旨 | Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their ...Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size and highly dynamic nature. Here we determined the structure of the asymmetric unit of the inner ring (IR monomer) at 3.73 Å resolution by single-particle cryo-electron microscopy, and created an atomic model of the intact IR consisting of 192 molecules of 8 nucleoporins. In each IR monomer, the Z-shaped Nup188-Nup192 complex in the middle layer is sandwiched by two approximately parallel rhomboidal structures in the inner and outer layers, while Nup188, Nup192 and Nic96 link all subunits to constitute a relatively stable IR monomer. In contrast, the intact IR is assembled by loose and instable interactions between IR monomers. These structures, together with previously reported structural information of IR, reveal two distinct interaction modes between IR monomers and extensive flexible connections in IR assembly, providing a structural basis for the stability and malleability of IR. |
リンク | Cell Res / PubMed:35301440 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.81 - 12.03 Å |
構造データ | EMDB-32643, PDB-7wo9: EMDB-32644: Cryo-EM structure of full-length Nup188 (multiBody refined N-terminal region) EMDB-32645: Cryo-EM structure of full-length Nup188 (multibody refined C-terminal region) EMDB-32653, PDB-7woo: EMDB-32658, PDB-7wot: EMDB-32662: Cryo-EM map of the inner ring dimer of the Saccharomyces cerevisiae nuclear pore complex EMDB-32663: Cryo-EM map of the intact inner ring of the Saccharomyces cerevisiae nuclear pore complex EMDB-32664: Cryo-EM map of the whole Saccharomyces cerevisiae nuclear pore complex |
由来 |
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キーワード | TRANSPORT PROTEIN / Nup188 / cryo-EM / Saccharomyces cerevisiae / nuclear pore complex / inner ring / protomer / monomer |