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-Structure paper
タイトル | Volta phase plate cryo-EM of the small protein complex Prx3. |
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ジャーナル・号・ページ | Nat Commun, Vol. 7, Page 10534, Year 2016 |
掲載日 | 2016年1月28日 |
著者 | Maryam Khoshouei / Mazdak Radjainia / Amy J Phillips / Juliet A Gerrard / Alok K Mitra / Jürgen M Plitzko / Wolfgang Baumeister / Radostin Danev / |
PubMed 要旨 | Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre ...Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination. |
リンク | Nat Commun / PubMed:26817416 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.4 Å |
構造データ | EMDB-3233: |
由来 |
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