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-Structure paper
Title | Volta phase plate cryo-EM of the small protein complex Prx3. |
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Journal, issue, pages | Nat Commun, Vol. 7, Page 10534, Year 2016 |
Publish date | Jan 28, 2016 |
![]() | Maryam Khoshouei / Mazdak Radjainia / Amy J Phillips / Juliet A Gerrard / Alok K Mitra / Jürgen M Plitzko / Wolfgang Baumeister / Radostin Danev / ![]() ![]() ![]() |
PubMed Abstract | Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre ...Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination. |
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Methods | EM (single particle) |
Resolution | 4.4 Å |
Structure data | ![]() EMDB-3233: |
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