EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	8 Å structure of the outer rings of the Xenopus laevis nuclear pore complex obtained by cryo-EM and AI.
ジャーナル・号・ページ	Protein Cell, Vol. 13, Issue 10, Page 760-777, Year 2022
掲載日	2022年1月11日
著者	Linhua Tai / Yun Zhu / He Ren / Xiaojun Huang / Chuanmao Zhang / Fei Sun /
PubMed 要旨	The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo- ...The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo-electron microscopic (cryo-EM) structure of the outer rings containing nuclear ring (NR) and cytoplasmic ring (CR) from the Xenopus laevis NPC, with local resolutions reaching 4.9 Å. With the aid of AlphaFold2, we managed to build a pseudoatomic model of the outer rings, including the Y complexes and flanking components. In this most comprehensive and accurate model of outer rings to date, the almost complete Y complex structure exhibits much tighter interaction in the hub region. In addition to two copies of Y complexes, each asymmetric subunit in CR contains five copies of Nup358, two copies of the Nup214 complex, two copies of Nup205 and one copy of newly identified Nup93, while that in NR contains one copy of Nup205, one copy of ELYS and one copy of Nup93. These in-depth structural features represent a great advance in understanding the assembly of NPCs.
リンク	Protein Cell / PubMed:35015240 / PubMed Central
手法	EM (単粒子)
解像度	7.8 - 8.9 Å
構造データ	31891 7vci EMDB-31891, PDB-7vci: Structure of Xenopus laevis NPC nuclear ring asymmetric unit 手法: EM (単粒子) / 解像度: 8.1 Å EMDB-31892: Cryo-EM structure of the nuclear ring asymmetric unit core of the Xenopus laevis nuclear pore complex 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-31893: Cryo-EM structure of nuclear ring asymmetric unit region of Xenopus laevis nuclear pore complex 手法: EM (単粒子) / 解像度: 8.6 Å 32056 7vop EMDB-32056, PDB-7vop: Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring subunit 手法: EM (単粒子) / 解像度: 8.7 Å EMDB-32060: Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring subunit core region 手法: EM (単粒子) / 解像度: 7.9 Å EMDB-32061: Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring Nup358 region 手法: EM (単粒子) / 解像度: 8.9 Å
由来	xenopus laevis (アフリカツメガエル)
キーワード	NUCLEAR PROTEIN / nuclear pore complex (核膜孔) / nuclear ring / asymmetric unit / cytoplasmic ring / cryo-EM (低温電子顕微鏡法) / Xenopus laevis (アフリカツメガエル)