EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural snapshots of V/A-ATPase reveal the rotary catalytic mechanism of rotary ATPases.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 1213, Year 2022
掲載日	2022年3月8日
著者	J Kishikawa / A Nakanishi / A Nakano / S Saeki / A Furuta / T Kato / K Mistuoka / K Yokoyama /
PubMed 要旨	V/A-ATPase is a motor protein that shares a common rotary catalytic mechanism with FF ATP synthase. When powered by ATP hydrolysis, the V domain rotates the central rotor against the AB hexamer, ...V/A-ATPase is a motor protein that shares a common rotary catalytic mechanism with FF ATP synthase. When powered by ATP hydrolysis, the V domain rotates the central rotor against the AB hexamer, composed of three catalytic AB dimers adopting different conformations (AB, AB, and AB). Here, we report the atomic models of 18 catalytic intermediates of the V domain of V/A-ATPase under different reaction conditions, determined by single particle cryo-EM. The models reveal that the rotor does not rotate immediately after binding of ATP to the V. Instead, three events proceed simultaneously with the 120˚ rotation of the shaft: hydrolysis of ATP in AB, zipper movement in AB by the binding ATP, and unzipper movement in AB with release of both ADP and Pi. This indicates the unidirectional rotation of V/A-ATPase by a ratchet-like mechanism owing to ATP hydrolysis in AB, rather than the power stroke model proposed previously for F-ATPase.
リンク	Nat Commun / PubMed:35260556 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 6.3 Å
構造データ	EMDB-31841: Nucleotide-free V/A-ATPase from Thermus thermophilus, State1 手法: EM (単粒子) / 解像度: 3.1 Å 31842 7vai EMDB-31842, PDB-7vai: V1EG of V/A-ATPase from Thermus thermophilus, state1-1 手法: EM (単粒子) / 解像度: 3.1 Å 31843 7vaj EMDB-31843, PDB-7vaj: Nucleotide-free V1EG domain of V/A-ATPase from Thermus thermophilus, state1-2 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-31844: Nucleotide-free V/A-ATPase from Thermus thermophilus, state2 手法: EM (単粒子) / 解像度: 4.7 Å 31845 7vak EMDB-31845, PDB-7vak: Nucleotide-free V1EG domain of V/A-ATPase from Thermus thermophilus, state2 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-31846: Nucleotide-free V/A-ATPase from Thermus thermophilus, state3 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-31847: V/A-ATPase from Thermus thermophilus, high ATP, state1-1 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-31848: V/A-ATPase ftom Thermus thermophilus, high ATP, state1-2 手法: EM (単粒子) / 解像度: 3.6 Å 31849 7val EMDB-31849, PDB-7val: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state1-1 手法: EM (単粒子) / 解像度: 3.1 Å 31850 7vam EMDB-31850, PDB-7vam: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state1-2 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-31851: V/A-ATPase from Thermus thermophilus, high ATP, state2-1 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-31852: V/A-ATPase from Thermus thermophilus, high ATP, state2-2 手法: EM (単粒子) / 解像度: 4.0 Å 31853 7van EMDB-31853, PDB-7van: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state2-1 手法: EM (単粒子) / 解像度: 3.0 Å 31854 7vao EMDB-31854, PDB-7vao: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state2-2 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-31855: V/A-ATPase from Thermus thermophilus, high ATP, state3-1 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-31856: V/A-ATPase from Thermus thermophilus, high ATP, state3-2 手法: EM (単粒子) / 解像度: 4.5 Å 31857 7vap EMDB-31857, PDB-7vap: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state2-2 手法: EM (単粒子) / 解像度: 3.0 Å 31858 7vaq EMDB-31858, PDB-7vaq: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state3-2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-31859: V/A-ATPase from Thermus thermophilus at low ATP concentration, state1 手法: EM (単粒子) / 解像度: 2.7 Å 31860 7var EMDB-31860, PDB-7var: V1EG domain of V/A-ATPase from Thermus thermophilus at low ATP concentration, state1-1 手法: EM (単粒子) / 解像度: 2.9 Å 31861 7vas EMDB-31861, PDB-7vas: V1EG domain of V/A-ATPase from Thermus thermophilus at low ATP concentration, state1-2 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-31862: V/A-ATPase from Thermus thermophilus at low ATP concentration, state2 手法: EM (単粒子) / 解像度: 3.3 Å 31863 7vat EMDB-31863, PDB-7vat: V1EG of V/A-ATPase from Thermus thermophilus at low ATP concentration, state2-1 手法: EM (単粒子) / 解像度: 3.2 Å 31864 7vau EMDB-31864, PDB-7vau: V1EG of V/A-ATPase from Thermus thermophilus at low ATP concentration, state2-2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-31865: V/A-ATPase from Thermus thermophilus at low ATP concentration, state3 手法: EM (単粒子) / 解像度: 3.6 Å 31866 7vav EMDB-31866, PDB-7vav: V1EG of V/A-ATPase from Thermus thermophilus at low ATP concentration, state3 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-31867: V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state1 手法: EM (単粒子) / 解像度: 2.7 Å 31868 7vaw EMDB-31868, PDB-7vaw: V1EG domain of V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state1-1 手法: EM (単粒子) / 解像度: 2.7 Å 31869 7vax EMDB-31869, PDB-7vax: V1EG of V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state1-2 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-31870: V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state2 手法: EM (単粒子) / 解像度: 3.3 Å 31871 7vay EMDB-31871, PDB-7vay: V1EG domain of V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-31872: V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state3 手法: EM (単粒子) / 解像度: 3.6 Å 31873 7vb0 EMDB-31873, PDB-7vb0: V1EG domain of V/A-ATPase from Thermus thermophilus at saturated ATP-gamma-S condition, state3 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体*YM
由来	thermus thermophilus hb8 (バクテリア)
キーワード	MOTOR PROTEIN / rotary ATPase / V-type ATPase / ATP synthase / Thermus thermophilus / chemo-mechanical coupling