+検索条件
-Structure paper
タイトル | Assembly status transition offers an avenue for activity modulation of a supramolecular enzyme. |
---|---|
ジャーナル・号・ページ | Elife, Vol. 10, Year 2021 |
掲載日 | 2021年12月13日 |
著者 | Yao Chen / Weiya Xu / Shuwei Yu / Kang Ni / Guangbiao She / Xiaodong Ye / Qiong Xing / Jian Zhao / Chengdong Huang / |
PubMed 要旨 | Nature has evolved many supramolecular proteins assembled in certain, sometimes even seemingly oversophisticated, morphological manners. The rationale behind such evolutionary efforts is often poorly ...Nature has evolved many supramolecular proteins assembled in certain, sometimes even seemingly oversophisticated, morphological manners. The rationale behind such evolutionary efforts is often poorly understood. Here, we provide atomic-resolution insights into how the dynamic building of a structurally complex enzyme with higher order symmetry offers amenability to intricate regulation. We have established the functional coupling between enzymatic activity and protein morphological states of glutamine synthetase (GS), an old multi-subunit enzyme essential for cellular nitrogen metabolism. Cryo-EM structure determination of GS in both the catalytically active and inactive assembly states allows us to reveal an unanticipated self-assembly-induced disorder-order transition paradigm, in which the remote interactions between two subcomplex entities significantly rigidify the otherwise structurally fluctuating active sites, thereby regulating activity. We further show in vivo evidences that how the enzyme morphology transitions could be modulated by cellular factors on demand. Collectively, our data present an example of how assembly status transition offers an avenue for activity modulation, and sharpens our mechanistic understanding of the complex functional and regulatory properties of supramolecular enzymes. |
リンク | Elife / PubMed:34898426 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.9 - 3.5 Å |
構造データ | EMDB-31711, PDB-7v4h: EMDB-31712, PDB-7v4i: EMDB-31713, PDB-7v4j: EMDB-31714, PDB-7v4k: EMDB-31715, PDB-7v4l: |
由来 |
|
キーワード | LIGASE / supramolecular enzyme / glutamine synthetase / Glycine max / PLANT PROTEIN / IMMUNE SYSTEM / Camellia sinensis |