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-Structure paper
タイトル | Cryo-EM structure of mycobacterial cytochrome bd reveals two oxygen access channels. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 4621, Year 2021 |
掲載日 | 2021年7月30日 |
著者 | Weiwei Wang / Yan Gao / Yanting Tang / Xiaoting Zhou / Yuezheng Lai / Shan Zhou / Yuying Zhang / Xiuna Yang / Fengjiang Liu / Luke W Guddat / Quan Wang / Zihe Rao / Hongri Gong / |
PubMed 要旨 | Cytochromes bd are ubiquitous amongst prokaryotes including many human-pathogenic bacteria. Such complexes are targets for the development of antimicrobial drugs. However, an understanding of the ...Cytochromes bd are ubiquitous amongst prokaryotes including many human-pathogenic bacteria. Such complexes are targets for the development of antimicrobial drugs. However, an understanding of the relationship between the structure and functional mechanisms of these oxidases is incomplete. Here, we have determined the 2.8 Å structure of Mycobacterium smegmatis cytochrome bd by single-particle cryo-electron microscopy. This bd oxidase consists of two subunits CydA and CydB, that adopt a pseudo two-fold symmetrical arrangement. The structural topology of its Q-loop domain, whose function is to bind the substrate, quinol, is significantly different compared to the C-terminal region reported for cytochromes bd from Geobacillus thermodenitrificans (G. th) and Escherichia coli (E. coli). In addition, we have identified two potential oxygen access channels in the structure and shown that similar tunnels also exist in G. th and E. coli cytochromes bd. This study provides insights to develop a framework for the rational design of antituberculosis compounds that block the oxygen access channels of this oxidase. |
リンク | Nat Commun / PubMed:34330928 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.79 - 2.87 Å |
構造データ | EMDB-30582, PDB-7d5i: EMDB-31302: |
化合物 | ChemComp-HEB: ChemComp-HDD: |
由来 |
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キーワード | OXIDOREDUCTASE / Mycobacterium smegmatis / electron transfer chain |