EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.
ジャーナル・号・ページ	Science, Vol. 371, Issue 6525, Year 2021
掲載日	2021年1月8日
著者	Rui Bai / Ruixue Wan / Chuangye Yan / Qi Jia / Jianlin Lei / Yigong Shi /
PubMed 要旨	Spliceosome remodeling, executed by conserved adenosine triphosphatase (ATPase)/helicases including Prp2, enables precursor messenger RNA (pre-mRNA) splicing. However, the structural basis for the ...Spliceosome remodeling, executed by conserved adenosine triphosphatase (ATPase)/helicases including Prp2, enables precursor messenger RNA (pre-mRNA) splicing. However, the structural basis for the function of the ATPase/helicases remains poorly understood. Here, we report atomic structures of Prp2 in isolation, Prp2 complexed with its coactivator Spp2, and Prp2-loaded activated spliceosome and the results of structure-guided biochemical analysis. Prp2 weakly associates with the spliceosome and cannot function without Spp2, which stably associates with Prp2 and anchors on the spliceosome, thus tethering Prp2 to the activated spliceosome and allowing Prp2 to function. Pre-mRNA is loaded into a featured channel between the N and C halves of Prp2, where Leu from the N half and Arg from the C half prevent backward sliding of pre-mRNA toward its 5'-end. Adenosine 5'-triphosphate binding and hydrolysis trigger interdomain movement in Prp2, which drives unidirectional stepwise translocation of pre-mRNA toward its 3'-end. These conserved mechanisms explain the coupling of spliceosome remodeling to pre-mRNA splicing.
リンク	Science / PubMed:33243853
手法	EM (単粒子)
解像度	2.5 - 3.2 Å
構造データ	30637 7dco EMDB-30637, PDB-7dco: Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom 手法: EM (単粒子) / 解像度: 2.5 Å 30638 7dcp EMDB-30638, PDB-7dcp: cryo-EM structure of the DEAH-box helicase Prp2 and coactivator Spp2 手法: EM (単粒子) / 解像度: 3.15 Å 30639 7dcq EMDB-30639, PDB-7dcq: cryo-EM structure of the DEAH-box helicase Prp2 手法: EM (単粒子) / 解像度: 2.9 Å 30640 7dcr EMDB-30640, PDB-7dcr: cryo-EM structure of the DEAH-box helicase Prp2 in complex with its coactivator Spp2 手法: EM (単粒子) / 解像度: 3.15 Å 30643 7dd3 EMDB-30643, PDB-7dd3: Cryo-EM structure of the pre-mRNA-loaded DEAH-box ATPase/helicase Prp2 in complex with Spp2 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸 ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	SPLICING / RNA splicing / spliceosome / Bact complex / Prp2 / Spp2 / ATPase/helicase / activation / DEAH-box ATPase/helicase