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-Structure paper
タイトル | Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. |
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ジャーナル・号・ページ | Science, Vol. 370, Issue 6521, Year 2020 |
掲載日 | 2020年12月4日 |
![]() | Shoucai Ma / Dmitry Lapin / Li Liu / Yue Sun / Wen Song / Xiaoxiao Zhang / Elke Logemann / Dongli Yu / Jia Wang / Jan Jirschitzka / Zhifu Han / Paul Schulze-Lefert / Jane E Parker / Jijie Chai / ![]() ![]() |
PubMed 要旨 | Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The effector ATR1 activates the ...Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme. |
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手法 | EM (単粒子) |
解像度 | 2.99 - 3.21 Å |
構造データ | EMDB-30449, PDB-7crb: EMDB-30450, PDB-7crc: ![]() EMDB-30451: EMDB-30579, PDB-7dfv: |
化合物 | ![]() ChemComp-ADP: ![]() ChemComp-ATP: |
由来 |
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![]() | PLANT PROTEIN / NADase / ETI / HR |